| Autor: |
Adnan M; Department of Bio-Health Technology, Kangwon National University, Chuncheon 24341, Korea. mdadnan1991.pharma@gmail.com.; Senior Scientist, Rentia Plant Factory, Chuncheon 24341, Korea. mdadnan1991.pharma@gmail.com., Nazim Uddin Chy M; Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh. nazim107282@gmail.com.; Drug Discovery, GUSTO A Research Group, Chittagong 4000, Bangladesh. nazim107282@gmail.com., Mostafa Kamal ATM; Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh. mostafa@pharm.iiuc.ac.bd., Azad MOK; Department of Bio-Health Technology, Kangwon National University, Chuncheon 24341, Korea. azadokalam@gmail.com.; Head of Research and Technology, Rentia Plant Factory, Chuncheon 24341, Korea. azadokalam@gmail.com., Paul A; Drug Discovery, GUSTO A Research Group, Chittagong 4000, Bangladesh. arka.bgctub@gmail.com.; Department of Microbiology, Jagannath University, Dhaka 1100, Bangladesh. arka.bgctub@gmail.com., Uddin SB; Ethnobotany and Pharmacognosy Lab, Department of Botany, University of Chittagong, Chittagong 4331, Bangladesh. bokhtear@cu.ac.bd., Barlow JW; Department of Chemistry, Royal College of Surgeons in Ireland, D02YN77 Dublin, Ireland. jambarlow@rcsi.ie., Faruque MO; Ethnobotany and Pharmacognosy Lab, Department of Botany, University of Chittagong, Chittagong 4331, Bangladesh. omf@cu.ac.bd., Park CH; Department of Bio-Health Technology, Kangwon National University, Chuncheon 24341, Korea. chpark@kangwon.ac.kr., Cho DH; Department of Bio-Health Technology, Kangwon National University, Chuncheon 24341, Korea. chodh@kangwon.ac.kr. |
| Abstrakt: |
Ophiorrhiza rugosa var. prostrata is one of the most frequently used ethnomedicinal plants by the indigenous communities of Bangladesh. This study was designed to investigate the antidiarrheal, anti-inflammatory, anthelmintic and antibacterial activities of the ethanol extract of O. rugosa leaves (EEOR). The leaves were extracted with ethanol and subjected to in vivo antidiarrheal screening using the castor oil-induced diarrhea, enteropooling, and gastrointestinal transit models. Anti-inflammatory efficacy was evaluated using the histamine-induced paw edema test. In parallel, in vitro anthelmintic and antibacterial activities were evaluated using the aquatic worm and disc diffusion assays respectively. In all three diarrheal models, EEOR (100, 200 and 400 mg/kg) showed obvious inhibition of diarrheal stool frequency, reduction of the volume and weight of the intestinal contents, and significant inhibition of intestinal motility. Also, EEOR manifested dose-dependent anti-inflammatory activity. Anthelmintic action was deemed significant ( P < 0.001) with respect to the onset of paralysis and helminth death. EEOR also resulted in strong zones of inhibition when tested against both Gram-positive and Gram-negative bacteria. GC-MS analysis identified 30 compounds within EEOR, and of these, 13 compounds documented as bioactive showed good binding affinities to M3 muscarinic acetylcholine, 5-HT3, tubulin and GlcN-6-P synthase protein targets in molecular docking experiments. Additionally, ADME/T and PASS analyses revealed their drug-likeness, likely safety upon consumption and possible pharmacological activities. In conclusion, our findings scientifically support the ethnomedicinal use and value of this plant, which may provide a potential source for future development of medicines. |
