| Autor: |
Godwin MA; Department of Nanotechnology, Noorul Islam Centre for Higher Education, Kumaracoil, Tamil Nadu 629180, India., Mahithashri K; Department of Nanotechnology, Noorul Islam Centre for Higher Education, Kumaracoil, Tamil Nadu 629180, India., Shiney OJ; Department of Nanotechnology, Noorul Islam Centre for Higher Education, Kumaracoil, Tamil Nadu 629180, India., Bhagat M; School of Biotechnology, University of Jammu, Jammu Tawi 180006, India., Praseetha PK; Department of Nanotechnology, Noorul Islam Centre for Higher Education, Kumaracoil, Tamil Nadu 629180, India. |
| Abstrakt: |
Besides numerous advantages, poor penetration, larger size and less efficient nanomaterials are the current challenges in nanomedicine-based therapies. Graphitic carbon nitride (g-C₃N₄) possesses all the constructive features to overcome the hurdles in cancer therapy. This is a detailed study on the prospects of utilizing g-C₃N₄ as a therapeutic agent and through this study it has been established that metal incorporations have improved their performance at in vitro levels. g-C₃N₄ nanomaterial was prepared by simple thermal decomposition process. The synthesized nanosheets were characterized by using UV-visible spectrometer, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and thermo-gravimetric analysis (TG/DTA). The incorporation of metal particles also has been confirmed by the above mentioned methods. Dose dependent cytotoxicity studies were performed on three different cell lines such as A549, PC-3 and MCF-7. The free radical scavenging activity of g-C₃N₄ nanosheets was promising using 1,1'-diphenyl-2-picrylhydrazyl (DPPH) assay. Cell scavenging activity of ˜45% with DPPH was observed at 100 μl concentrations of Ag/g-C₃N₄, Zn/g-C₃N₄ and g-C₃N₄. The cytotoxicity and free radical scavenging of cancer cell lines was better with metal incorporated g-C₃N₄ than their bare counterparts. Hence the study of these thin sheets of nanomaterial is encouraging to be explored as one of the future tools for biomedical therapeutics. |
