Application of ELISA to Retail Survey of Aflatoxin B 1 in Peanut Butter.

Autor: Ram BP; Department of Food Science and Human Nutrition and Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824 and National Peanut Research Laboratory, United States Department of Agriculture, Agricultural Research Service, Dawson, Georgia 31742., Hart LP; Department of Food Science and Human Nutrition and Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824 and National Peanut Research Laboratory, United States Department of Agriculture, Agricultural Research Service, Dawson, Georgia 31742., Cole RJ; Department of Food Science and Human Nutrition and Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824 and National Peanut Research Laboratory, United States Department of Agriculture, Agricultural Research Service, Dawson, Georgia 31742., Pestka JJ; Department of Food Science and Human Nutrition and Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824 and National Peanut Research Laboratory, United States Department of Agriculture, Agricultural Research Service, Dawson, Georgia 31742.
Jazyk: angličtina
Zdroj: Journal of food protection [J Food Prot] 1986 Oct; Vol. 49 (10), pp. 792-795.
DOI: 10.4315/0362-028X-49.10.792
Abstrakt: A simple procedure was devised for the routine screening of aflatoxin B 1 (AFB 1 ) in peanut butter using enzyme-linked immunosorbent assay (ELISA). Peanut butter samples (5 g) were artificially contaminated with AFB 1 and extracted by blending with 25 ml of 55% methanol and 10 ml of hexane. The extract was filtered and aqueous filtrate analyzed by a direct competitive ELISA. Recovery of AFB 1 added to peanut butter samples ranged from 85 to 112%, with an average inter-well coefficient of variation of 18.4%. The inter-assay coefficient of variation was 22.7%. Using this procedure, only 3 of 63 commercial samples of peanut butter had detectable levels (>5.0 μg/kg) of AFB 1 .
Databáze: MEDLINE
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