An extended core nanocoax pillar architecture for enhanced molecular detection.

Autor: D'Imperio LA; Department of Physics, Boston College, Chestnut Hill, MA 02467, United States., Valera AE; Department of Biology, Boston College, Chestnut Hill, MA 02467, United States., Naughton JR; Department of Physics, Boston College, Chestnut Hill, MA 02467, United States., Archibald MM; Department of Biology, Boston College, Chestnut Hill, MA 02467, United States., Merlo JM; Department of Physics, Boston College, Chestnut Hill, MA 02467, United States., Connolly TJ; Department of Biology, Boston College, Chestnut Hill, MA 02467, United States., Burns MJ; Department of Physics, Boston College, Chestnut Hill, MA 02467, United States., Chiles TC; Department of Biology, Boston College, Chestnut Hill, MA 02467, United States., Naughton MJ; Department of Physics, Boston College, Chestnut Hill, MA 02467, United States. Electronic address: naughton@bc.edu.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2019 Jun 01; Vol. 134, pp. 83-89. Date of Electronic Publication: 2019 Mar 22.
DOI: 10.1016/j.bios.2019.03.045
Abstrakt: Biosensors that incorporate nanomaterials and nanofabrication techniques enable molecular detection of chemical and biological macromolecules with a high degree of specificity and ultrasensitivity. Here, we present a novel fabrication process that yields a nanostructure capable of detecting biological macromolecules. The extended core nanocoax (ECC) structure builds on a previously reported nanocoaxial-based sensor. The fabrication of the device incorporates an extended inner pillar, with controllable extension above the annulus and into the surrounding solution. This new design eliminates structural constraints inherent in the original nanocoax architecture. We also provide results demonstrating improvement in biosensing capability. Specifically, we show the capability of the new architecture to detect the B subunit of the Vibrio cholerae toxin at improved sensitivity (100 pg/ml) in comparison to optical enzyme-linked immunosorbant assay (1 ng/ml) and previously reported coaxial nanostructures (2 ng/ml).
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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