Preparation and in vitro absorption studies of a novel polysaccharide‑iron (III) complex from Flammulina velutipes.

Autor: Cheng C; Department of Food Quality and Safety, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, China., Huang DC; Department of Food Quality and Safety, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, China., Zhao LY; College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China., Cao CJ; Department of Food Quality and Safety, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, China., Chen GT; Department of Food Quality and Safety, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, China. Electronic address: cpucgt@cpu.edu.cn.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2019 Jul 01; Vol. 132, pp. 801-810. Date of Electronic Publication: 2019 Apr 03.
DOI: 10.1016/j.ijbiomac.2019.04.015
Abstrakt: Iron is essential in fundamental bioactivities, so it makes sense to improve the efficiency of iron on epithelial transport. In this work, a novel polysaccharide‑iron(III) complex (FVP-Fe(III)) was prepared from Flammulina velutipes with a specific structure. The FVP-Fe(III) had a molecular weight of 15.13 kDa with a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9. In the vitro digestion model, the complex could maintain better solubility and steady of iron than FeSO 4 . In the cell assay, FVP-Fe(III) showed lower cytotoxicity and better absorption. The transport amount of FVP-Fe(III) was 1.5-fold of FeSO 4 at same concentration and 1.8-fold of FeSO 4 at same time. The transport was mediated by the peptide transporter (pepT1) active transport pathway and the efflux of the sample was mainly mediated by multidrug-resistance proteins (MRP) transporter. The results of this study suggested that the polysaccharide obtained from F. velutipes could be developed a new kind of iron delivery for further study.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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