Determination of uric acid in serum using an optical sensor based on binuclear Pd(II) 2-pyrazinecarboxamide-bipyridine doped in a sol gel matrix.

Autor: Hashem SG; Ain Shams University, Department of chemistry, Faculty of Science, Abbassia, 11566 Cairo, Egypt., Elsaady MM; Ain Shams University, Department of chemistry, Faculty of Science, Abbassia, 11566 Cairo, Egypt., Afify HG; Ain Shams University, Department of chemistry, Faculty of Science, Abbassia, 11566 Cairo, Egypt., Omer WE; Institute of Nanoscience and Nanotechnology, Kafrelsheikh University, Egypt., Youssef AO; Ain Shams University, Department of chemistry, Faculty of Science, Abbassia, 11566 Cairo, Egypt., El-Kemary M; Institute of Nanoscience and Nanotechnology, Kafrelsheikh University, Egypt., Attia MS; Ain Shams University, Department of chemistry, Faculty of Science, Abbassia, 11566 Cairo, Egypt. Electronic address: Mohd_mostafa@sci.asu.edu.eg.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2019 Jul 01; Vol. 199, pp. 89-96. Date of Electronic Publication: 2019 Feb 13.
DOI: 10.1016/j.talanta.2019.02.056
Abstrakt: A new highly green luminescent binuclear palladium 2-pyrazinecarboxamide-bipyridine complex [Pd(pyc)(bpy)] was prepared and characterized. The binuclear Pd(pyc)(bpy) complex doped in sol-gel matrix has a strong luminescence intensity at 547 nm with λ ex = 330 nm in water The method depends on the quenching of the luminescence intensity of the binuclear Pd(pyc)(bpy) complex at 547 nm by different concentrations of uric acid. The remarkable quenching of the luminescence intensity of the binuclear Pd(pyc)(bpy) complex, doped in a sol-gel matrix, by uric acid was successfully used for the determination of uric acid in serum samples of patients with hypouricemia disease. The calibration plot was achieved over the concentration 3.9 × 10 -9 to 1.2 × 10 -4 mol L -1 uric acid with a correlation coefficient of 0.9 and a detection limit of 1.8 × 10 -10 mol L -1 . The method was used satisfactorily for the assessment of the uric acid in a number of serum samples collected from various patients with Hypouricemia disease.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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