Bioluminescent aptamer-based solid-phase microassay to detect lung tumor cells in plasma.
| Autor: | Bashmakova EE; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS, Akademgorodok 50/50, 660036 Krasnoyarsk, Russia; Siberian Federal University, Svobodny pr. 79, 660041 Krasnoyarsk, Russia., Krasitskaya VV; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS, Akademgorodok 50/50, 660036 Krasnoyarsk, Russia., Zamay GS; State Medical University named after V.F. Voyno-Yasenetsky, Partizana Zheleznyaka St. 1, 660022 Krasnoyarsk, Russia., Zamay TN; State Medical University named after V.F. Voyno-Yasenetsky, Partizana Zheleznyaka St. 1, 660022 Krasnoyarsk, Russia., Frank LA; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS, Akademgorodok 50/50, 660036 Krasnoyarsk, Russia; Siberian Federal University, Svobodny pr. 79, 660041 Krasnoyarsk, Russia. Electronic address: lfrank@yandex.ru. |
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| Jazyk: | angličtina |
| Zdroj: | Talanta [Talanta] 2019 Jul 01; Vol. 199, pp. 674-678. Date of Electronic Publication: 2019 Mar 05. |
| DOI: | 10.1016/j.talanta.2019.03.030 |
| Abstrakt: | Two high-affinity DNA aptamers for lung tumor cells were applied as biospecific elements in bioluminescent assay of patient blood. The oligonucleotide complementary to the 5' end of both aptamers carrying either biotin or Ca 2+ -regulated photoprotein obelin was used to form a sandwich-type analytical complex on the surfaces of magnetic streptavidin-activated microspherical particles. Clinical blood samples from cases of morphologically confirmed lung cancer and control samples were analyzed applying the developed assay. From the receiver operator curve (ROC) analysis, the chosen threshold value as clinical decision limit offers the sensitivity of 91.5% and the specificity of 75% (p < 0.001). The area under ROC curve with the value of 0.901 distinguishes well between the two groups under investigation. (Copyright © 2019 Elsevier B.V. All rights reserved.) |
| Databáze: | MEDLINE |
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