Antibacterial and cytotoxic assessment of poly (methyl methacrylate) based hybrid nanocomposites.

Autor: Sathya S; Biofouling and Thermal Ecology Section, Bhabha Atomic Research Centre, Kalpakkam 603 102, Tamil Nadu, India; Bioengineering and Drug Design Lab, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, Tamil Nadu, India., Murthy PS; Biofouling and Thermal Ecology Section, Bhabha Atomic Research Centre, Kalpakkam 603 102, Tamil Nadu, India; Homi Bhabha National Institute, Anushaktinagar, Mumbai, Maharashtra 400094, India., Devi VG; Bioengineering and Drug Design Lab, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, Tamil Nadu, India., Das A; Surface and Nanoscience Division, Indira Gandhi Centre for Atomic Research, Kalpakkam 603 102, Tamil Nadu, India; Homi Bhabha National Institute, Anushaktinagar, Mumbai, Maharashtra 400094, India., Anandkumar B; Corrosion Science and Technology Division, Indira Gandhi Centre for Atomic Research, Kalpakkam 603 102, Tamil Nadu, India., Sathyaseelan VS; Biofouling and Thermal Ecology Section, Bhabha Atomic Research Centre, Kalpakkam 603 102, Tamil Nadu, India., Doble M; Bioengineering and Drug Design Lab, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, Tamil Nadu, India. Electronic address: mukeshd@iitm.ac.in., Venugopalan VP; Homi Bhabha National Institute, Anushaktinagar, Mumbai, Maharashtra 400094, India. Electronic address: vpvenu@barc.gov.in.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2019 Jul; Vol. 100, pp. 886-896. Date of Electronic Publication: 2019 Mar 19.
DOI: 10.1016/j.msec.2019.03.053
Abstrakt: Poly (methyl methacrylate) (PMMA) is an extensively used implant material in biomedical devices. Biofilm formation creates issues in PMMA-based biomedical implants, while emergence of drug resistant pathogens poses an additional complication. Hence development of surfaces that resist bacterial colonisation is extremely desirable. In this context, nanomaterials are among the potential choices. In the present work, nanocomposites (NCs) were developed by incorporation of chemically synthesized nanoparticles of CuO, cetyl trimethyl ammonium bromide (CTAB) capped CuO and ZnO (singly and in combination) in PMMA. The efficacy of these NCs was assessed against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) bacteria which are prevalent in many implant-associated infections. Results revealed species-specific response of the bacteria towards nanomaterials. CuO NC (0.1% (w/v)) was more effective against E. coli, while CTAB capped CuO NC and ZnO NC were very effective against S. aureus. Furthermore, combination of nanoparticles improved efficacy of nanocomposites against both the bacterial species. In vitro cytotoxicity assay using L6 myoblast cell line showed that all NCs at 0.1% (w/v) were biocompatible, showing >85% cell viability. The present study suggests that combination of NPs is a promising option to combat implant infection by multiple organisms.
(Copyright © 2019. Published by Elsevier B.V.)
Databáze: MEDLINE
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