A novel evolutionary conserved mechanism of RNA stability regulates synexpression of primordial germ cell-specific genes prior to the sex-determination stage in medaka.

Autor: Herpin A; INRA, UR1037 Fish Physiology and Genomics, Rennes, France.; University of Wuerzburg, Physiological Chemistry, Biocenter, Wuerzburg, Germany., Schmidt C; University of Wuerzburg, Physiological Chemistry, Biocenter, Wuerzburg, Germany., Kneitz S; University of Wuerzburg, Physiological Chemistry, Biocenter, Wuerzburg, Germany., Gobé C; UMR BDR, INRA, ENVA, Université Paris Saclay, Jouy en Josas, France., Regensburger M; University of Wuerzburg, Physiological Chemistry, Biocenter, Wuerzburg, Germany., Le Cam A; INRA, UR1037 Fish Physiology and Genomics, Rennes, France., Montfort J; INRA, UR1037 Fish Physiology and Genomics, Rennes, France., Adolfi MC; University of Wuerzburg, Physiological Chemistry, Biocenter, Wuerzburg, Germany., Lillesaar C; University of Wuerzburg, Physiological Chemistry, Biocenter, Wuerzburg, Germany., Wilhelm D; University of Melbourne, Department of Anatomy & Neuroscience, Parkville, Victoria, Australia., Kraeussling M; University of Wuerzburg, Physiological Chemistry, Biocenter, Wuerzburg, Germany., Mourot B; INRA, UR1037 Fish Physiology and Genomics, Rennes, France., Porcon B; INRA, UR1037 Fish Physiology and Genomics, Rennes, France., Pannetier M; UMR BDR, INRA, ENVA, Université Paris Saclay, Jouy en Josas, France., Pailhoux E; UMR BDR, INRA, ENVA, Université Paris Saclay, Jouy en Josas, France., Ettwiller L; University of Heidelberg, Centre for Organismal Studies (COS), Department of Developmental Biology, Heidelberg, Germany., Dolle D; University of Heidelberg, Centre for Organismal Studies (COS), Department of Developmental Biology, Heidelberg, Germany., Guiguen Y; INRA, UR1037 Fish Physiology and Genomics, Rennes, France., Schartl M; University of Wuerzburg, Physiological Chemistry, Biocenter, Wuerzburg, Germany.; Comprehensive Cancer Center Mainfranken, University Hospital, Wuerzburg, Germany.; Hagler Institute for Advanced Study and Department of Biology, Texas A&M University, College Station, Texas, United States of America.
Jazyk: angličtina
Zdroj: PLoS biology [PLoS Biol] 2019 Apr 04; Vol. 17 (4), pp. e3000185. Date of Electronic Publication: 2019 Apr 04 (Print Publication: 2019).
DOI: 10.1371/journal.pbio.3000185
Abstrakt: Dmrt1 is a highly conserved transcription factor, which is critically involved in regulation of gonad development of vertebrates. In medaka, a duplicate of dmrt1-acting as master sex-determining gene-has a tightly timely and spatially controlled gonadal expression pattern. In addition to transcriptional regulation, a sequence motif in the 3' UTR (D3U-box) mediates transcript stability of dmrt1 mRNAs from medaka and other vertebrates. We show here that in medaka, two RNA-binding proteins with antagonizing properties target this D3U-box, promoting either RNA stabilization in germ cells or degradation in the soma. The D3U-box is also conserved in other germ-cell transcripts, making them responsive to the same RNA binding proteins. The evolutionary conservation of the D3U-box motif within dmrt1 genes of metazoans-together with preserved expression patterns of the targeting RNA binding proteins in subsets of germ cells-suggest that this new mechanism for controlling RNA stability is not restricted to fishes but might also apply to other vertebrates.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje