Prediction of the Quantity and Purity of an Antibody Capture Process in Real Time.

Autor: Walch N; Austrian Centre of Industrial Biotechnology, Muthgasse 18, A-1190, Vienna, Austria.; Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna Muthgasse 18, A-1190, Vienna, Austria., Scharl T; Austrian Centre of Industrial Biotechnology, Muthgasse 18, A-1190, Vienna, Austria.; Institute of Statistics, University of Natural Resources and Life Sciences Vienna, Peter-Jordan-Straße 82, A-1190, Vienna, Austria., Felföldi E; Austrian Centre of Industrial Biotechnology, Muthgasse 18, A-1190, Vienna, Austria.; Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna Muthgasse 18, A-1190, Vienna, Austria., Sauer DG; Austrian Centre of Industrial Biotechnology, Muthgasse 18, A-1190, Vienna, Austria.; Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna Muthgasse 18, A-1190, Vienna, Austria., Melcher M; Austrian Centre of Industrial Biotechnology, Muthgasse 18, A-1190, Vienna, Austria.; Institute of Statistics, University of Natural Resources and Life Sciences Vienna, Peter-Jordan-Straße 82, A-1190, Vienna, Austria., Leisch F; Austrian Centre of Industrial Biotechnology, Muthgasse 18, A-1190, Vienna, Austria.; Institute of Statistics, University of Natural Resources and Life Sciences Vienna, Peter-Jordan-Straße 82, A-1190, Vienna, Austria., Dürauer A; Austrian Centre of Industrial Biotechnology, Muthgasse 18, A-1190, Vienna, Austria.; Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna Muthgasse 18, A-1190, Vienna, Austria., Jungbauer A; Austrian Centre of Industrial Biotechnology, Muthgasse 18, A-1190, Vienna, Austria.; Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna Muthgasse 18, A-1190, Vienna, Austria.
Jazyk: angličtina
Zdroj: Biotechnology journal [Biotechnol J] 2019 Jul; Vol. 14 (7), pp. e1800521. Date of Electronic Publication: 2019 May 20.
DOI: 10.1002/biot.201800521
Abstrakt: Regulatory recommendations for quality by design instead of quality by testing raise increasing interest in new sensor technologies. An online monitoring system for downstream processes is developed, which is based on an array of online detectors. Besides standard detectors (UV, pH, and conductivity), our chromatographic workstation is equipped with a fluorescence and a mid-infrared spectrometer, a light scattering, and a refractive index detector. The combination of these sensors enables the prediction of specific protein concentration and various purity attributes, such as high molecular weight impurities, DNA and host cell protein content during the elution phase of a chromatographic antibody capture process. Prediction models solely based on online signals are set up providing real-time predictions. No mechanistic models or information about the chromatographic runs is used. These predictions allow online pooling decisions replacing time- and labor-intensive laboratory measurements. Different process variations, such as changes in the column load or elution buffer, are introduced to test the predictive power of the models. Extrapolation of the models worked well when the column load is changed, whereas model adjustment is necessary when the elution conditions are changed considerably.
(© 2019 The Authors. Biotechnology Journal Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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