IgE antibodies from schistosomiasis patients to recognize epitopes in potato apyrase.

Autor:	Gusmão MADN; Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brasil., Emídio NB; Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brasil., Marconato DG; Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brasil., Farani PSG; Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brasil., Maniezzi LF; Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brasil., Coelho PMZ; Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brasil., Oliveira RC; Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brasil., Vasconcelos EG; Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brasil., Faria-Pinto P; Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brasil.
Jazyk:	angličtina
Zdroj:	Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2019 Mar 28; Vol. 52, pp. e20180139. Date of Electronic Publication: 2019 Mar 28.
DOI:	10.1590/0037-8682-0139-2018
Abstrakt:	Introduction: High percentages of structural identity and cross-immunoreactivity have been reported between potato apyrase and Schistosoma mansoni ATP diphosphohydrolase (SmATPDases) isoforms, showing the existence of particular epitopes shared between these proteins. Methods: Potato apyrase was employed using ELISA, western blot, and mouse immunization methods to verify IgE reactivity. Results: Most of the schistosomiasis patient's (75%) serum was seropositive for potato apyrase and this protein was recognized using western blotting, suggesting that parasite and plant proteins share IgE-binding epitopes. C57BL/6 mice immunized with potato apyrase showed increased IgE antibody production. Conclusions: Potato apyrase and SmATPDases have IgE-binding epitopes.
Databáze:	MEDLINE
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