B cell profiling in malaria reveals expansion and remodelling of CD11c+ B cell subsets.

Autor:	Sundling C; Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.; Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden., Rönnberg C; Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.; Department of Microbiology, Tumor, and Cell Biology, Karolinska Institutet, Stockholm, Sweden.; Department of Clinical Microbiology, Karolinska University Hospital, Huddinge, Stockholm, Sweden., Yman V; Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden., Asghar M; Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.; Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden., Jahnmatz P; Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.; Mabtech AB, Stockholm, Sweden., Lakshmikanth T; Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, Solna, Sweden., Chen Y; Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, Solna, Sweden., Mikes J; Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, Solna, Sweden., Forsell MN; Division of Infection & Immunology, Department of Clinical Microbiology, Umeå University, Umeå, Sweden., Sondén K; Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.; Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden., Achour A; Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.; Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden.; Science for Life Laboratory, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden., Brodin P; Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, Solna, Sweden.; Department of Newborn Medicine, Karolinska University Hospital, Solna, Sweden., Persson KE; Department of Microbiology, Tumor, and Cell Biology, Karolinska Institutet, Stockholm, Sweden.; Department of Laboratory Medicine, Lund University, Skåne University Hospital, Lund, Sweden., Färnert A; Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.; Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden.
Jazyk:	angličtina
Zdroj:	JCI insight [JCI Insight] 2019 Apr 02; Vol. 5. Date of Electronic Publication: 2019 Apr 02.
DOI:	10.1172/jci.insight.126492
Abstrakt:	Humoral immunity is important in limiting clinical disease in malaria, yet the longitudinal B cell response to infection remains unclear. We performed a 1-year prospective study in patients treated for acute P. falciparum malaria for the first time, or with previous exposure to the disease. Using an unbiased exploratory approach with mass cytometry, followed by targeted flow cytometry, we found that ~80% of mature B cells that proliferated in response to acute infection expressed CD11c. Only ~40% of CD11c+ B cells displayed an atypical B cell phenotype, with the remaining cells primarily made up of activated- and resting memory B cells. The CD11c+ B cells expanded rapidly following infection, with previous exposure to malaria resulting in a significantly larger increase compared to individuals with primary infection. This was attributed to an expansion of switched CD11c+ B cells that was absent in primary infected individuals. The rate of contraction of the CD11c+ B cell compartment was independent of previous exposure to malaria and displayed a slow decay with a half-life of ~300 days. Collectively, these results identify CD11c as a marker of B cells responding to malaria and further highlight differences in primary- and secondary B cell responses during infection.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu