Clinical evaluation and cost analysis of a Trioplex real-time PCR assay for the detection and differentiation of herpes simplex virus 1 and 2 in cutaneous and mucocutaneous lesions.
| Autor: | Navidad J; City of Milwaukee Health Department Laboratory, Milwaukee, Wisconsin, USA., Pfotenhauer B; City of Milwaukee Health Department Laboratory, Milwaukee, Wisconsin, USA., Leigh N; City of Milwaukee Health Department Laboratory, Milwaukee, Wisconsin, USA., Maas E; City of Milwaukee Health Department Laboratory, Milwaukee, Wisconsin, USA.; University of Wisconsin - Milwaukee, Milwaukee, Wisconsin, USA., Gradus S; City of Milwaukee Health Department Laboratory, Milwaukee, Wisconsin, USA., Bhattacharyya S; City of Milwaukee Health Department Laboratory, Milwaukee, Wisconsin, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of medical microbiology [J Med Microbiol] 2019 May; Vol. 68 (5), pp. 748-754. Date of Electronic Publication: 2019 Apr 02. |
| DOI: | 10.1099/jmm.0.000971 |
| Abstrakt: | Purpose: Herpes simplex virus (HSV) is a common lifelong sexually transmitted infection. HSV-1 typically manifests as oral cold sores, while HSV-2 is more traditionally associated with sexual transmission and infection. We have developed a real-time PCR (Trioplex) for the simultaneous detection of HSV-1 and -2 and the bacterial phage internal control (IC) MS2. Methodology: To determine the performance of the Trioplex method and resolve discrepancies, 178 clinical specimens from cutaneous and mucocutaneous sources were tested using 3 different methods; virus culture with direct fluorescent antibody (DFA) immunostaining, Trioplex and a commercially available HSV analyte-specific reagent (ASR). Results: HSV Trioplex was significantly more sensitive than virus culture (89 and 67 % HSV 1/2, respectively) and comparable to the commercial assay (P<0.001). Cost analysis revealed that the Trioplex reduced cost by 80 % compared to cell culture. Conclusions: The implementation of the HSV Trioplex improved the detection turnaround time from 3-10 days to 2.5 h, thus streamlining Herpes detection, improving sensitivity and reducing laboratory costs. |
| Databáze: | MEDLINE |
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