Modulation of the resistance to norfloxacin in Staphylococcus aureus by Bauhinia forficata link.

Autor: de Sousa JN; Laboratory of Research in Microbiology, Federal University of Piauí, Teresina, PI, Brazil., de Oliveira ABM; Laboratory of Research in Microbiology, Federal University of Piauí, Teresina, PI, Brazil., Ferreira AK, Silva E; Laboratory of Research in Microbiology, Federal University of Piauí, Teresina, PI, Brazil.; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, PI, Brazil., de Sousa LMS; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, PI, Brazil., França Rocha MC; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, PI, Brazil., de JP, Júnior S; Laboratory of Genetics of Microorganisms, Federal University of Paraiba (UFPB), João Pessoa, Brazil., William Kaatz G; Department of Medicine, Division of Infectious Diseases, Wayne State University School of Medicine, Detroit, MI, USA., da Silva Almeida JRG; Department of Medicine, Division of Infectious Diseases, Wayne State University School of Medicine, Detroit, MI, USA., de Souza JSN; Nucleus of Studies and Research of Medicinal Plants, Federal University of the São Francisco Valley, Juazeiro, BA, Brazil., Medeiros Barreto H; Laboratory of Research in Microbiology, Federal University of Piauí, Teresina, PI, Brazil.
Jazyk: angličtina
Zdroj: Natural product research [Nat Prod Res] 2021 Feb; Vol. 35 (4), pp. 681-685. Date of Electronic Publication: 2019 Apr 02.
DOI: 10.1080/14786419.2019.1590714
Abstrakt: Microdilution assays were performed in order to evaluate the antimicrobial activity of the ethanoic extract from the leaves of Bauhinia forficate (EEBF) against different microorganisms. The extract did not present inner antimicrobial activities against the tested strains. However, EEBF was able to modulate the norfloxacin-resistance against Staphylococcus aureus SA1199-B that overproduce the NorA efflux pump, once sub-inhibitory concentrations of EEBF reduced the minimal inhibitory concentratio of the norfloxacin in 87.5%. This modulatory effect was also found when the antibiotic was replaced by ethidium bromide, suggesting that EEBF acts probably by inhibition of NorA, allowing the antibiotic accumulation intracellularly, and making the line more sensitive. These results point out the EEBF potential as a source of NorA inhibitors that could be used in combination with norfloxacin for treatment of infections caused by multidrug-resistant S. aureus .
Databáze: MEDLINE