Terminomics Methodologies and the Completeness of Reductive Dimethylation: A Meta-Analysis of Publicly Available Datasets.

Autor: Hurtado Silva M; Proteomics Core Facility and School of Life Sciences, Faculty of Science, University of Technology Sydney, Broadway NSW 2007, Australia. Mariella.I.Hurtado@alumni.uts.edu.au., Berry IJ; Proteomics Core Facility and School of Life Sciences, Faculty of Science, University of Technology Sydney, Broadway NSW 2007, Australia. iain.berry@uts.edu.au.; The ithree Institute, Faculty of Science, University of Technology Sydney, Broadway NSW 2007, Australia. iain.berry@uts.edu.au., Strange N; Proteomics Core Facility and School of Life Sciences, Faculty of Science, University of Technology Sydney, Broadway NSW 2007, Australia. natalie.strange@student.uts.edu.au., Djordjevic SP; The ithree Institute, Faculty of Science, University of Technology Sydney, Broadway NSW 2007, Australia. steven.djordjevic@uts.edu.au., Padula MP; Proteomics Core Facility and School of Life Sciences, Faculty of Science, University of Technology Sydney, Broadway NSW 2007, Australia. matthew.padula@uts.edu.au.
Jazyk: angličtina
Zdroj: Proteomes [Proteomes] 2019 Mar 29; Vol. 7 (2). Date of Electronic Publication: 2019 Mar 29.
DOI: 10.3390/proteomes7020011
Abstrakt: Methods for analyzing the terminal sequences of proteins have been refined over the previous decade; however, few studies have evaluated the quality of the data that have been produced from those methodologies. While performing global N-terminal labelling on bacteria, we observed that the labelling was not complete and investigated whether this was a common occurrence. We assessed the completeness of labelling in a selection of existing, publicly available N-terminomics datasets and empirically determined that amine-based labelling chemistry does not achieve complete labelling and potentially has issues with labelling amine groups at sequence-specific residues. This finding led us to conduct a thorough review of the historical literature that showed that this is not an unexpected finding, with numerous publications reporting incomplete labelling. These findings have implications for the quantitation of N-terminal peptides and the biological interpretations of these data.
Databáze: MEDLINE
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