Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing.

Autor: Ignatov KB; All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences, Moscow, Russia.; Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia., Blagodatskikh KA; Evrogen JSC, Moscow, Russia.; Pirogov Russian National Research Medical University, Moscow, Russia., Shcherbo DS; Pirogov Russian National Research Medical University, Moscow, Russia., Kramarova TV; The Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden., Monakhova YA; All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences, Moscow, Russia.; Syntol JSC, Moscow, Russia., Kramarov VM; All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences, Moscow, Russia.; Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2019 Apr 01; Vol. 14 (4), pp. e0210374. Date of Electronic Publication: 2019 Apr 01 (Print Publication: 2019).
DOI: 10.1371/journal.pone.0210374
Abstrakt: Fragmentation of DNA is the very important first step in preparing nucleic acids for next-generation sequencing. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. This method generates double-stranded DNA fragments that are suitable for direct use in NGS library construction and allows the elimination of the additional step of reparation of DNA ends.
Competing Interests: K.A. Blagodatskikh is employed by Evrogen JSC. Y.A. Monakhova is employed by Syntol JSC. T.V. Kramarova is currently employed by Anocca AB. SD polymerase used in this project is the subject of a patent application (US20160145588, EP2981609). This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials.
Databáze: MEDLINE
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