Ethanol Extract of Securidaca longipedunculata Induces Apoptosis in Brain Tumor (U87) Cells.

Autor: Ngulde SI; Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary, University of Maiduguri, PMB 1069, Maiduguri, Borno State, Nigeria., Sandabe UK; Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary, University of Maiduguri, PMB 1069, Maiduguri, Borno State, Nigeria., Abounader R; Departments of Microbiology, Immunology and Cancer Biology, University of Virginia, PO Box 800168, Charlottesville, VA 22908, USA., Dawson TK; Chemistry Department, University of Virginia, PO Box 800168, Charlottesville, VA 22908, USA., Zhang Y; Departments of Microbiology, Immunology and Cancer Biology, University of Virginia, PO Box 800168, Charlottesville, VA 22908, USA., Iliya I; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Maiduguri, PMB 1069, Maiduguri, Borno State, Nigeria., Hussaini IM; Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Maiduguri, PMB 1069, Maiduguri, Borno State, Nigeria.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2019 Feb 26; Vol. 2019, pp. 9826590. Date of Electronic Publication: 2019 Feb 26 (Print Publication: 2019).
DOI: 10.1155/2019/9826590
Abstrakt: Incidence of cancer is estimated to be on the increase and current anticancer drugs are characterized by narrow margin of safety and side effects. There is the need to explore new drugs especially from plants since plants serve as major source of drugs. Securidaca longipedunculata Fresen plant is called the mother of all medicines in northern Nigeria and is used traditionally in the treatment of cancers by most traditional medicine practitioners in the region. This study is aimed at evaluating the anticancer activity of the plant extract using U87 brain tumor cell line. Ethanol extract of its root bark was prepared and fractionated by silica gel column chromatography. In vitro activity of the extract and fractions were assessed on the viability of U87 malignant brain tumor cell line by using hemacytometer, annexin V-PE and 7AAD flow cytometry and western blot detection of Poly-ADP-Ribose-Polymerase (PARP) cleavage. The results showed that the extract significantly (p<0.01) inhibited proliferation of U87 cell line with IC 50 of 20.535 μ g/ml. Apoptosis was induced by the extract (41.53 ± 10.33%) and the polar fraction (47.3 ± 2.7%) via cleavage of PARP. It was concluded that the ethanol extract of S. longipedunculata root bark inhibited proliferation of U87 cell line and induced apoptosis by cleavage of PARP, thus supporting folkloric use of the plant in the management of cancers.
Databáze: MEDLINE
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