The endogenous hydrogen sulfide generating system regulates ovulation.

Autor: Estienne A; Centre de Recherche en Reproduction et Fertilité, Faculty of Veterinary Medicine, University of Montreal, St-Hyacinthe, J2S 7C6, QC, Canada., Portela VM; Centre de Recherche en Reproduction et Fertilité, Faculty of Veterinary Medicine, University of Montreal, St-Hyacinthe, J2S 7C6, QC, Canada., Choi Y; Department of Obstetrics and Gynecology, Chandler Medical Center, 800 Rose Street, University of Kentucky, Lexington, KY, 40536-0298, USA., Zamberlam G; Centre de Recherche en Reproduction et Fertilité, Faculty of Veterinary Medicine, University of Montreal, St-Hyacinthe, J2S 7C6, QC, Canada., Boerboom D; Centre de Recherche en Reproduction et Fertilité, Faculty of Veterinary Medicine, University of Montreal, St-Hyacinthe, J2S 7C6, QC, Canada., Roussel V; Centre de Recherche en Reproduction et Fertilité, Faculty of Veterinary Medicine, University of Montreal, St-Hyacinthe, J2S 7C6, QC, Canada., Meinsohn MC; Centre de Recherche en Reproduction et Fertilité, Faculty of Veterinary Medicine, University of Montreal, St-Hyacinthe, J2S 7C6, QC, Canada., Brännström M; Department of Obstetrics and Gynecology, University of Gothenburg, 405 30, Gothenburg, Sweden; Stockholm IVF, 112 81, Stockholm, Sweden., Curry TE Jr; Department of Obstetrics and Gynecology, Chandler Medical Center, 800 Rose Street, University of Kentucky, Lexington, KY, 40536-0298, USA., Jo M; Department of Obstetrics and Gynecology, Chandler Medical Center, 800 Rose Street, University of Kentucky, Lexington, KY, 40536-0298, USA., Price CA; Centre de Recherche en Reproduction et Fertilité, Faculty of Veterinary Medicine, University of Montreal, St-Hyacinthe, J2S 7C6, QC, Canada. Electronic address: christopher.price@umontreal.ca.
Jazyk: angličtina
Zdroj: Free radical biology & medicine [Free Radic Biol Med] 2019 Jul; Vol. 138, pp. 43-52. Date of Electronic Publication: 2019 Mar 28.
DOI: 10.1016/j.freeradbiomed.2019.03.028
Abstrakt: The generation of free-radicals such as nitric oxide has been implicated in the regulation of ovarian function, including ovulation. Tissues that generate nitric oxide typically generate another free-radical gas, hydrogen sulfide (H 2 S), although little is known about the role of H 2 S in ovarian function. The hypothesis of this study was that H 2 S regulates ovulation. Treatment with luteinizing hormone (LH) increased the levels of mRNA and protein of the H 2 S generating enzyme cystathionine γ-lyase (CTH) in granulosa cells of mice and humans in vivo and in vitro. Pharmacological inhibition of H 2 S generating enzymes reduced the number of follicles ovulating in mice in vivo and in vitro, and this inhibitory action was reversed by cotreatment with a H 2 S donor. Addition of a H 2 S donor to cultured mouse granulosa cells increased basal and LH-dependent abundance of mRNA encoding amphiregulin, betacellulin and tumor necrosis alpha induced protein 6, proteins important for cumulus expansion and follicle rupture. Inhibition of CTH activity reduced abundance of mRNA encoding matrix metalloproteinase-2 and -9 and tissue-type plasminogen activator, and cotreatment with the H 2 S donor increased the levels of these mRNA above those stimulated by LH alone. We conclude that the H 2 S generating system plays an important role in the propagation of the preovulatory cascade and rupture of the follicle at ovulation.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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