Variability and directionality of inferior olive neuron dendrites revealed by detailed 3D characterization of an extensive morphological library.

Autor: Vrieler N; Department of Neurobiology, Institute of Life Sciences and Edmond and Lily Safra Center for Brain Sciences, Hebrew University, Jerusalem, Israel., Loyola S; Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences, Amsterdam, The Netherlands.; Department of Neuroscience, Erasmus MC, Rotterdam, The Netherlands., Yarden-Rabinowitz Y; Department of Neurobiology, Institute of Life Sciences and Edmond and Lily Safra Center for Brain Sciences, Hebrew University, Jerusalem, Israel., Hoogendorp J; Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences, Amsterdam, The Netherlands., Medvedev N; Computational Neuroscience Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa, 904-0495, Japan., Hoogland TM; Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences, Amsterdam, The Netherlands.; Department of Neuroscience, Erasmus MC, Rotterdam, The Netherlands., De Zeeuw CI; Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences, Amsterdam, The Netherlands.; Department of Neuroscience, Erasmus MC, Rotterdam, The Netherlands., De Schutter E; Computational Neuroscience Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa, 904-0495, Japan., Yarom Y; Department of Neurobiology, Institute of Life Sciences and Edmond and Lily Safra Center for Brain Sciences, Hebrew University, Jerusalem, Israel., Negrello M; Department of Neuroscience, Erasmus MC, Rotterdam, The Netherlands., Torben-Nielsen B; Neurolinx Research Institute, La Jolla, CA, USA., Uusisaari MY; Neuronal Rhythms in Movement Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa, 904-0495, Japan. uusisaari@oist.jp.
Jazyk: angličtina
Zdroj: Brain structure & function [Brain Struct Funct] 2019 May; Vol. 224 (4), pp. 1677-1695. Date of Electronic Publication: 2019 Mar 30.
DOI: 10.1007/s00429-019-01859-z
Abstrakt: The inferior olive (IO) is an evolutionarily conserved brain stem structure and its output activity plays a major role in the cerebellar computation necessary for controlling the temporal accuracy of motor behavior. The precise timing and synchronization of IO network activity has been attributed to the dendro-dendritic gap junctions mediating electrical coupling within the IO nucleus. Thus, the dendritic morphology and spatial arrangement of IO neurons governs how synchronized activity emerges in this nucleus. To date, IO neuron structural properties have been characterized in few studies and with small numbers of neurons; these investigations have described IO neurons as belonging to two morphologically distinct types, "curly" and "straight". In this work we collect a large number of individual IO neuron morphologies visualized using different labeling techniques and present a thorough examination of their morphological properties and spatial arrangement within the olivary neuropil. Our results show that the extensive heterogeneity in IO neuron dendritic morphologies occupies a continuous range between the classically described "curly" and "straight" types, and that this continuum is well represented by a relatively simple measure of "straightness". Furthermore, we find that IO neuron dendritic trees are often directionally oriented. Combined with an examination of cell body density distributions and dendritic orientation of adjacent IO neurons, our results suggest that the IO network may be organized into groups of densely coupled neurons interspersed with areas of weaker coupling.
Databáze: MEDLINE
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