Protein complexes associated with β-catenin differentially influence the differentiation profile of neonatal and adult CD8 + T cells.

Autor: Hernández-Acevedo GN; Centro de Investigación en Dinámica Celular, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico., López-Portales OH; Centro de Investigación en Dinámica Celular, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico., Gutiérrez-Reyna DY; Centro de Investigación en Dinámica Celular, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico., Cuevas-Fernández E; Centro de Investigación en Dinámica Celular, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico., Kempis-Calanis LA; Centro de Investigación en Dinámica Celular, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico., Labastida-Conde RG; Centro de Investigación en Dinámica Celular, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico., Aguilar-Luviano OB; Centro de Investigación en Dinámica Celular, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico., Ramírez-Pliego O; Centro de Investigación en Dinámica Celular, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico., Spicuglia S; Aix-Marseille University, Inserm, TAGC, UMR1090, Marseille, France.; Equipe Labélisée Ligue Contre le Cancer, Marseille, France., Lino-Alfaro B; Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, Irapuato, Mexico., Chagolla-López A; Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, Irapuato, Mexico., González-de la Vara LE; Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, Irapuato, Mexico., Santana MA; Centro de Investigación en Dinámica Celular, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
Jazyk: angličtina
Zdroj: Journal of cellular physiology [J Cell Physiol] 2019 Aug; Vol. 234 (10), pp. 18639-18652. Date of Electronic Publication: 2019 Mar 28.
DOI: 10.1002/jcp.28502
Abstrakt: The canonical Wnt signaling pathway is a master cell regulator involved in CD8 + T cell proliferation and differentiation. In human CD8 + T cells, this pathway induces differentiation into memory cells or a "stem cell memory like" population, which is preferentially present in cord blood. To better understand the role of canonical Wnt signals in neonatal or adult blood, we compared the proteins associated with β-catenin, in nonstimulated and Wnt3a-stimulated human neonatal and adult naive CD8 + T cells. Differentially recruited proteins established different complexes in adult and neonatal cells. In the former, β-catenin-associated proteins were linked to cell signaling and immunological functions, whereas those of neonates were linked to proliferation and metabolism. Wnt3a stimulation led to the recruitment and overexpression of Wnt11 in adult cells and Wnt5a in neonatal cells, suggesting a differential connexion with planar polarity and Wnt/Ca 2+ noncanonical pathways, respectively. The chromatin immunoprecipitation polymerase chain reaction β-catenin was recruited to a higher level on the promoters of cell renewal genes in neonatal cells and of differentiation genes in those of adults. We found a preferential association of β-catenin with CBP in neonatal cells and with p300 in the adult samples, which could be involved in a higher self-renewal capacity of the neonatal cells and memory commitment in those of adults. Altogether, our results show that different proteins associated with β-catenin during Wnt3a activation mediate a differential response of neonatal and adult human CD8 + T cells.
(© 2019 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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