Reference intervals for serum cystatin C and serum creatinine in an adult sub-Saharan African population.

Autor: Edinga-Melenge BE; Department of Biochemistry, Centre Pasteur of Cameroon, Yaoundé, Cameroon.; 2Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon., Yakam AT; 3Ebebda District Hospital, Centre Regional Delegation, Ministry of Public Health, Ebebda, Cameroon., Nansseu JR; 4Department for the Control of Disease, Epidemics and Pandemics, Ministry of Public Health, Yaoundé, Cameroon.; 5Department of Public Health, Faculty of Medicine and Biomedical Sciences of the University of Yaoundé I, PO Box 1364, Yaoundé, Cameroon., Bilong C; Department of Biochemistry, Centre Pasteur of Cameroon, Yaoundé, Cameroon., Belinga S; Department of Biochemistry, Centre Pasteur of Cameroon, Yaoundé, Cameroon., Minkala E; Department of Biochemistry, Centre Pasteur of Cameroon, Yaoundé, Cameroon., Noudjeu PA; Department of Biochemistry, Centre Pasteur of Cameroon, Yaoundé, Cameroon., Ondhoua M; Department of Biochemistry, Centre Pasteur of Cameroon, Yaoundé, Cameroon., Kokola SW; Department of Biochemistry, Centre Pasteur of Cameroon, Yaoundé, Cameroon., Ama Moor VJ; 2Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon.; 6Laboratory of Biochemistry, Yaoundé University Teaching Hospital, Yaoundé, Cameroon., Ashuntantang G; 7Cardiology and Nephrology Unit, Yaoundé General Hospital, Yaoundé, Cameroon.; 8Department of Internal Medicine and Specialties, Faculty of Medicine and Biomedical Sciences of the University of Yaoundé I, Yaoundé, Cameroon.
Jazyk: angličtina
Zdroj: BMC clinical pathology [BMC Clin Pathol] 2019 Mar 18; Vol. 19, pp. 4. Date of Electronic Publication: 2019 Mar 18 (Print Publication: 2019).
DOI: 10.1186/s12907-019-0086-7
Abstrakt: Background: Serum cystatin C (SCysC) and serum creatinine (SCr) are two biomarkers used in common practice to estimate the glomerular filtration rate (GFR). For SCysC and SCr to be used in a given population, normal values need to be determined to better assess patients. This study aimed to determine SCysC and SCr reference intervals (RIs) in a Cameroonian adult population and factors susceptible of influencing them.
Methods: We carried-out a cross-sectional study from November 2016 to May 2017 in Yaoundé, Cameroon. Participants were Cameroonians aged 18 years and above, residing inside the country and found in good health at study inclusion. SCysC and SCr were determined by particle-enhanced turbidimetric immunoassay standardized against the ERM-DA471/IFCC reference material and by the IDMS reference modified Jaffe kinetic method, respectively. RIs were determined using the 2.5th and 97.5th percentiles and their respective 90% confidence intervals (CIs). The quantile regression served to identify potential factors likely influencing SCysC and SCr values.
Results: We included 381 subjects comprising 49.1% females.. RIs for SCysC varied between 0.57 (90%CI: 0.50-0.60) and 1.03 mg/L (90%CI: 1.00-1.10) for females, and from 0.70 (90%CI: 0.60-0.70) to 1.10 mg/L (90%CI: 1.10-1.20) for males. Concerning SCr, its RIs ranged from 0.58 (90%CI: 0.54-0.61) to 1.08 mg/dL (90%CI: 1.02-1.21) for females, and from 0.74 (90%CI: 0.70-0.80) to 1.36 mg/dL (90%CI: 1.30-1.45) for males. Men had significantly higher SCysC and SCr values than women ( p  <  0.001). Likewise, subjects aged 50 years and above had higher SCysC values in comparison to younger age groups (p <  0.001), which was not the case for SCr values ( p  = 0.491). Moreover, there was a positive and significant correlation between SCysC and SCr in women (ρ = 0.55, p  < 0.001), in men (ρ = 0.39, p  < 0.001) and globally (ρ = 0.58; p < 0.001). Furthermore, the sex influenced both biomarkers' values across all quantile regression models while age and body surface area (BSA) influenced them inconsistently.
Conclusion: This study has determined serum cystatin C and serum creatinine reference intervals in an adult Cameroonian population, whose interpretations might take into account the patient's sex and to a certain extent, his/her age and/or BSA.
Competing Interests: We were delivered an ethical clearance by the Ethics Committee for Human Health Research of the Ministry of Public Health, Cameroon (Ref: CE N° 0057/CRERSHC/2017) in addition to authorizations from administrative authorities of Yaoundé. All aspects and procedures in relation to the study were extensively presented and explained to each potential participant; we included only those who volunteered to be enrolled; they signed a consent form accordingly.Not applicable!The authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Databáze: MEDLINE
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