Label-free impedimetric immunosensor based on arginine-functionalized gold nanoparticles for detection of DHEAS, a biomarker of pediatric adrenocortical carcinoma.

Autor: Lima D; Department of Chemistry, Universidade Estadual de Ponta Grossa, Av. General Carlos Cavalcanti, 4748, 84030-900 Ponta Grossa, Paraná, Brazil., Inaba J; Department of Chemistry, Universidade Estadual de Ponta Grossa, Av. General Carlos Cavalcanti, 4748, 84030-900 Ponta Grossa, Paraná, Brazil., Clarindo Lopes L; Department of Chemistry, Universidade Estadual de Ponta Grossa, Av. General Carlos Cavalcanti, 4748, 84030-900 Ponta Grossa, Paraná, Brazil., Calaça GN; Department of Chemistry, Instituto Federal do Paraná, Rodovia PR 323, KM 310, 87507-014 Umuarama, Paraná, Brazil., Los Weinert P; Department of Chemistry, Universidade Estadual de Ponta Grossa, Av. General Carlos Cavalcanti, 4748, 84030-900 Ponta Grossa, Paraná, Brazil., Lenzi Fogaça R; Department of Basic Pathology, Universidade Federal do Paraná, Av. Coronel Francisco H. dos Santos, 100, 81530-000 Curitiba, Paraná, Brazil., Ferreira de Moura J; Department of Basic Pathology, Universidade Federal do Paraná, Av. Coronel Francisco H. dos Santos, 100, 81530-000 Curitiba, Paraná, Brazil., Magalhães Alvarenga L; Department of Basic Pathology, Universidade Federal do Paraná, Av. Coronel Francisco H. dos Santos, 100, 81530-000 Curitiba, Paraná, Brazil., Cavalcante de Figueiredo B; Department of Community Health, Universidade Federal do Paraná, Rua Padre Camargo, 261, 80069-240 Curitiba, Paraná, Brazil; Instituto de Pesquisa Pelé Pequeno Príncipe, Av. Silva Jardim, 1632, 80250-060 Curitiba, Paraná, Brazil., Wohnrath K; Department of Chemistry, Universidade Estadual de Ponta Grossa, Av. General Carlos Cavalcanti, 4748, 84030-900 Ponta Grossa, Paraná, Brazil., Andrade Pessôa C; Department of Chemistry, Universidade Estadual de Ponta Grossa, Av. General Carlos Cavalcanti, 4748, 84030-900 Ponta Grossa, Paraná, Brazil. Electronic address: christiana.pessoa@pq.cnpq.br.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2019 May 15; Vol. 133, pp. 86-93. Date of Electronic Publication: 2019 Mar 06.
DOI: 10.1016/j.bios.2019.02.063
Abstrakt: Pediatric adrenocortical carcinoma (pACC) is a rare and aggressive malignancy of high occurrence in Southern Brazil. pACC is characterized by the usual overproduction of dehydroepiandrosterone sulfate (DHEAS), whose detection in serum or plasma can be effective to the early diagnosis of the disease. Therefore, the present paper reports, for the first time, the construction and application of a label-free impedimetric immunosensor to detect DHEAS, which was based on the modification of an oxidized glassy carbon electrode with arginine-functionalized gold nanoparticles (AuNPs-ARG) and anti-DHEA IgM antibodies (ox-GCE/AuNPs-ARG/IgM). AuNPs-ARG was synthesized by a green route, and characterized by UV-VIS spectroscopy, FTIR, TEM, DLS, and XRD. The construction of ox-GCE/AuNPs-ARG/IgM was optimized through factorial design and response surface methodology. Cyclic voltammetry and electrochemical impedance spectroscopy measurements were employed to characterize the optimized immunosensor. The DHEAS detection principle was based on the variation of charge transfer resistance (∆R ct ) relative to the Fe(CN) 6 4-/3- electrochemical probe after immunoassays in the presence of the biomarker. A linear relationship between ∆R ct and DHEAS concentration was verified in the range from 10.0 to 110.0 µg dL -1 , with a LOD of 7.4 µg dL -1 . Besides the good sensitivity, the immunosensor displayed accuracy, stability, and specificity to detect DHEAS. The promising analytical performance of ox-GCE/AuNPs-ARG/IgM was confirmed by quantifying DHEAS in real patient plasma samples, with results that were comparable to the reference chemiluminescence assay. Our results suggest that the presented immunosensor can find clinical applications in the early diagnosis of pACC and to monitor DHEAS levels in other adrenal pathologies.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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