Mitochondrial affectation, DNA damage and AChE inhibition induced by Salvia officinalis essential oil on Aedes aegypti larvae.

Autor: Castillo-Morales RM; Centro de Investigaciones en Enfermedades Tropicales - CINTROP, Facultad de Salud, Escuela de Medicina, Departamento de Ciencias Básicas, Universidad Industrial de Santander, Bucaramanga, Santander, Colombia., Carreño Otero AL; Laboratorio de Química Orgánica y Biomolecular-LQOBio, Facultad de Ciencias, Escuela de Química, Centro de Investigaciónes en Enfermedades Tropicales - CINTROP, Facultad de Salud, Escuela de Medicina, Departamento de Ciencias Básicas, Universidad Industrial de Santander, Bucaramanga, Colombia. Electronic address: auliseca@uis.edu.co., Mendez-Sanchez SC; Grupo de Investigación en Bioquímica y Microbiología-GIBIM, Escuela de Química, Universidad Industrial de Santander, Bucaramanga, Colombia. Electronic address: scmendez@uis.edu.co., Da Silva MAN; Laboratório de Morfologia e Fisiologia de Culicidae e Chironomidae, Universidade Federal do Paraná, Departamento de Zoología, Pós-graduação em Entomología, PO Box 19020, 81531-980 Curitiba, Paraná, Brazil. Electronic address: mnavarro@ufpr.br., Stashenko EE; Centro de Investigación en Biomoléculas-CIBIMOL, Centro Nacional de Investigación para la Agroindustrialización de Plantas Aromáticas y Medicinales Tropicales - CENIVAM, Escuela de Química, Universidad Industrial de Santander, Bucaramanga, Santander, Colombia. Electronic address: elena@tucan.uis.edu.co., Duque JE; Centro de Investigaciones en Enfermedades Tropicales - CINTROP, Facultad de Salud, Escuela de Medicina, Departamento de Ciencias Básicas, Universidad Industrial de Santander Parque Tecnológico y de Investigaciones Guatiguara, Km 2 Vía El Refugio, Piedecuesta, Santander, Colombia. Electronic address: jonedulu@uis.edu.co.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2019 Jul; Vol. 221, pp. 29-37. Date of Electronic Publication: 2019 Mar 21.
DOI: 10.1016/j.cbpc.2019.03.006
Abstrakt: The aim of this research study was to understand the mechanism of action of Salvia officinalis (Lamiaceae) essential oil (EO) on Aedes aegypti larvae. We evaluated the effect on DNA damage, acetylcholinesterase (AChE) inhibition and mitochondrial enzymatic alterations. The major components were analyzed in silico using OSIRIS and Molispiration free software. Aedes aegypti DNA was extracted from mosquito larvae between third (L3) and fourth (L4) instars to determine the DNA fragmentation or degradation at S. officinalis EO lethal concentrations (LC 10 , LC 20 , LC 50 , and LC 90 ). DNA integrity was assessed in both LCs in larvae treated for 24 h and in larvae homogenized with EO; we also assessed purified DNA larvae by a densitometric analysis. The AChE inhibition was quantified in protein larvae L3-L4 following Ellman's method and the enzymatic activities related to the mitochondrial respiratory chain of mitochondrial proteins was estimated by spectrophotometry. In silico analysis of 1,8-cineol and of α-thujone, major EO components, showed that they were highly permeable in biological membranes without mutagenic risks. Alterations in the integrity of DNA were observed in larvae exposed and homogenized with S. officinalis EO. The EO induced an AChE inhibition of 37 ± 2.6% to IC 50 . On the other hand, mitochondrial bioenergetics suggest that EO inhibits electrons entry to the respiratory chain, via Complex II. AChE activity alteration causes mortality of individuals, by blocking the insect cholinergic functions. These results indicate that EO affects the integrity of DNA, the mitochondrial respiration chain and the AChE activity.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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