Effect of Sodium Lauryl Sulfate (SLS) on Primary Human Gingival Fibroblasts in an In Vitro Wound Healing Model.

Autor: Chuang AH; Department of Clinical Investigation, Dwight D. Eisenhower Army Medical Center, 7 Ave. Bldg 38705, Fort Gordon, GA., Bordlemay J; Department of Clinical Investigation, Dwight D. Eisenhower Army Medical Center, 7 Ave. Bldg 38705, Fort Gordon, GA., Goodin JL; Department of Clinical Investigation, Dwight D. Eisenhower Army Medical Center, 7 Ave. Bldg 38705, Fort Gordon, GA., McPherson JC; Department of Clinical Investigation, Dwight D. Eisenhower Army Medical Center, 7 Ave. Bldg 38705, Fort Gordon, GA.
Jazyk: angličtina
Zdroj: Military medicine [Mil Med] 2019 Mar 01; Vol. 184 (Suppl 1), pp. 97-101.
DOI: 10.1093/milmed/usy332
Abstrakt: Objectives: Sodium lauryl sulfate (SLS) is a surfactant used to decrease the surface tension of water. Most commercially available dentifrices contain 0.5-2.0% SLS. This study investigated the potential effect of SLS on oral wound healing using primary human gingival fibroblasts (HGFs).
Methods: HGFs cells were grown in12-well culture plates in DMEM medium. A 3 mm wound was created on confluent HGFs. The cells were challenged with 0 (the control group), 0.01, 0.02, 0.03, 0.04, or 0.05% SLS-containing media once daily for 2 minutes. The cells were stained on day 0, 2, 4, 6 and 8. The percent of wound fill area was measured.
Results: On day 2, 4, 6, and 8, the wound fill of the control group (0% SLS) was 15, 35, 67 and 98%, respectively; at 0.01% SLS, it was 10, 20, 65 and 84%; at 0.02%, it was 7, 10, 15 and 25%; at 0.03% SLS, it was only 5% and 8% on day 2 and 4.
Conclusion: Our results showed a dose- and time-dependent inhibition on HGFs wound fill by SLS; however, future in vivo studies are needed to validate if our in vitro findings using SLS-free dentifrices to avoid the potential delay of wound healing.
(Published by Oxford University Press on behalf of the Association of Military Surgeons of the United States 2019.)
Databáze: MEDLINE
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