Ribosomal mutation in helix 32 of 18S rRNA alters fidelity of eukaryotic translation start site selection.

Autor: Antony A C; School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, India.; Homi Bhabha National Institute (HBNI), Mumbai, India., Ram AK; School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, India.; Homi Bhabha National Institute (HBNI), Mumbai, India., Dutta K; School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, India.; Homi Bhabha National Institute (HBNI), Mumbai, India., Alone PV; School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, India.; Homi Bhabha National Institute (HBNI), Mumbai, India.
Jazyk: angličtina
Zdroj: FEBS letters [FEBS Lett] 2019 Apr; Vol. 593 (8), pp. 852-867. Date of Electronic Publication: 2019 Apr 02.
DOI: 10.1002/1873-3468.13369
Abstrakt: The 40S ribosome plays a critical role in start codon selection. To gain insights into the role of its 18S rRNA in start codon selection, a suppressor screen was performed that suppressed the preferential UUG start codon recognition (Suppressor of initiation codon: Sui - phenotype) associated with the eIF5 G31R mutant. The C1209U mutation in helix h32 of 18S rRNA was found to suppress the Sui - and Gcn - (failure to derepress GCN4 expression) phenotype of the eIF5 G31R mutant. The C1209U mutation suppressed Sui - and Gcd - (constitutive derepression of GCN4 expression) phenotype of eIF2β S264Y , eIF1 K60E , and eIF1A-ΔC mutation. We propose that the C1209U mutation in 40S ribosomal may perturb the premature head rotation in 'Closed/P IN ' state and enhance the stringency of translation start site selection.
(© 2019 Federation of European Biochemical Societies.)
Databáze: MEDLINE
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