| Autor: |
De Almeida CV; Department of Surgery and Translational Medicine, University of Florence, 50134 Florence, Italy. almeida.cv@gmail.com., Lulli M; Department of Experimental and Clinical Biomedical Sciences 'Mario Serio', University of Florence, 50134 Florence, Italy. matteo.lulli@unifi.it., di Pilato V; Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy. vincenzo.dipilato@unifi.it., Schiavone N; Department of Experimental and Clinical Biomedical Sciences 'Mario Serio', University of Florence, 50134 Florence, Italy. nicola.schiavone@unifi.it., Russo E; Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy. edda.russo@unifi.it., Nannini G; Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy. giulia.nannini@unifi.it., Baldi S; Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy. simone.baldi1@stud.unifi.it., Borrelli R; Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy. rossella.borrelli92@gmail.com., Bartolucci G; Department of Neurosciences, Psychology, Drug Research and Child Health Section of Pharmaceutical and Nutraceutical Sciences University of Florence, 50139 Florence, Italy. gianluca.bartolucci@unifi.it., Menicatti M; Department of Neurosciences, Psychology, Drug Research and Child Health Section of Pharmaceutical and Nutraceutical Sciences University of Florence, 50139 Florence, Italy. marta.menicatti@unifi.it., Taddei A; Department of Surgery and Translational Medicine, University of Florence, 50134 Florence, Italy. antonio.taddei@unifi.it., Ringressi MN; Department of Surgery and Translational Medicine, University of Florence, 50134 Florence, Italy. marianovella.ringressi@unifi.it., Niccolai E; Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy. elena.niccolai@unifi.it., Prisco D; Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy. domenico.prisco@unifi.it., Rossolini GM; Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy. gianmaria.rossolini@unifi.it.; Department of Microbiology and Virology Unit, Florence Careggi University Hospital, 50134 Florence, Italy. gianmaria.rossolini@unifi.it., Amedei A; Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy. amedeo.amedei@unifi.it.; Department of Biomedicine, Azienda Ospedaliera Universitaria Careggi (AOUC), 50134 Florence, Italy. amedeo.amedei@unifi.it. |
| Abstrakt: |
The metabolites produced by the host's gut microbiota have an important role in the maintenance of intestinal homeostasis, but can also act as toxins and induce DNA damage in colorectal epithelial cells increasing the colorectal cancer (CRC) chance. In this scenario, the impact of some of the components of the natural human gastrointestinal microbiota, such as Enterococcus faecalis ( E. faecalis ), at the onset of CRC progression remains controversial. Since under dysbiotic conditions it could turn into a pathogen, the aim of this study was to compare the effect of E. faecalis ' strains (isolated from CRC patients and healthy subjects' stools) on the proliferation of different colorectal cells lines. First, we isolated and genotyping characterized the Enterococcus faecalis ' strains. Then, we analyzed the proliferation index (by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay) of three tumor and one normal intestinal cell lines, previously exposed to E. faecalis strains pre-cultured medium. Stool samples of CRC patients demonstrated a reduced frequency of E. faecalis compared to healthy subjects. In addition, the secreted metabolites of E. faecalis ' strains, isolated from healthy donors, decreased the human ileocecal adenocarcinoma cell line HCT-8 and human colon carcinoma cell line HCT-116 cell proliferation without effects on human colorectal adenocarcinoma cell line SW620 and on normal human diploid cell line CLR-1790. Notably, the metabolites of the strains isolated from CRC patients did not influence the cell growth of CRC cell lines. Our results demonstrated a new point of view in the investigation of E. faecalis ' role in CRC development, which raises awareness of the importance of not only associating the presence/absence of a unique microorganism, but also in defining the specific characteristics of the different investigated strains. |
