| Autor: |
Omosa LK; Department of Chemistry, University of Nairobi, Nairobi, Kenya., Mbogo GM; Department of Chemistry, University of Nairobi, Nairobi, Kenya., Korir E; Department of Chemistry, University of Nairobi, Nairobi, Kenya., Omole R; Department of Chemical Science and Technology, Technical University of Kenya, Nairobi, Kenya., Seo EJ; Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany., Yenesew A; Department of Chemistry, University of Nairobi, Nairobi, Kenya., Heydenreich M; Institute of Chemistry, University of Potsdam, Potsdam, Germany., Midiwo JO; Department of Chemistry, University of Nairobi, Nairobi, Kenya., Efferth T; Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany. |
| Abstrakt: |
In our continuous search for cytotoxic compounds from the genus Zanthoxylum, chromatographic separation of the MeOH/CH 2 Cl 2 (1:1) extract of Z. chalybeum yielded one new alkamide; 4-(isoprenyloxy)-3-methoxy-3,4-deoxymethylenedioxyfagaramide ( 1 ) and a known one; fagaramide ( 2 ). Similarly, from the MeOH/CH 2 Cl 2 (1:1) extract of the stem bark of Z. parachanthum four known compounds; canthin-6-one ( 3 ), dihydrochelerythrine ( 4 ), lupeol ( 5 ) and sesamin ( 6 ) were isolated. Characterization of the structures of these compounds was achieved using spectroscopic techniques (NMR and MS). Using resazurin reduction assay 1 , 3 and 6 displayed moderate cytotoxicity with IC 50 values below 50 μM against the drug sensitive CCRF-CEM and multidrug-resistant CEM/ADR5000 leukemia cell lines. It is interesting to note that 3 was more active than the standard drug, doxorubicin against CEM/ADR5000 leukemia cells. Compounds 3 and 6 showed good selectivity on leukemia cells than normal cells. In future studies 3 should be tested against a panel of drug resistant human cells. |
