[Low concentrations of hepatitis C virus RNA in serologically mild infection.]

Autor:	Yaroslavtseva NG; National Medical Research Center of Hematology, 125167, Moscow, Russian Federation., Tikhomirov DS; National Medical Research Center of Hematology, 125167, Moscow, Russian Federation., Nikolaeva LI; D.I. Ivanovsky Institute of Virology «National Research Center of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya», 123098, Moscow, Russian Federation., Dedova AV; D.I. Ivanovsky Institute of Virology «National Research Center of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya», 123098, Moscow, Russian Federation., Ovchinnikova EN; National Medical Research Center of Hematology, 125167, Moscow, Russian Federation., Misko ON; National Medical Research Center of Hematology, 125167, Moscow, Russian Federation., Romanova TY; National Medical Research Center of Hematology, 125167, Moscow, Russian Federation., Makhnovskiy PI; D.I. Ivanovsky Institute of Virology «National Research Center of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya», 123098, Moscow, Russian Federation., Grishechkin AE; D.I. Ivanovsky Institute of Virology «National Research Center of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya», 123098, Moscow, Russian Federation., Tupoleva TA; National Medical Research Center of Hematology, 125167, Moscow, Russian Federation.
Jazyk:	ruština
Zdroj:	Voprosy virusologii [Vopr Virusol] 2019; Vol. 64 (1), pp. 30-35.
DOI:	10.18821/0507-4088-2019-64-1-30-35
Abstrakt:	Occult HCV infection (OCI) provides significant interest recently. HCV RNA in this case can be detected not in plasma, but in blood cells and/or in liver tissue. In case of antibody genesis impairment anti-HCV detection may lead to negative or "uncertain" result. The aim of the study was to estimate infection type in blood donors and patients with hematological diseases by exploration of samples with uncertain anti-HCV detection results. Blood samples of 30 180 potential blood donors' and 4322 patients with hematological diseases were tested. Comparative analysis of wide pattern of HCV markers was performed. 33 blood donors and 42 patients were enrolled in follow-up examination. Uncertain results of Anti-HCV detection in donors' samples were in 0.18% of cases. Follow-up examination of 33 donors provided discordant results using immunochemiluminescence assay and ELISA. 15.2% donors' samples contained HCV RNA in low concentration. Follow-up observation of 42 patients with incomplete antiviral antibody pattern showed HCV RNA presence in 40.5% cases (21.4% high viremia and 19.0% low viremia). Samples with low RNA concentration contained low titers of anti-core antibodies. Samples with high titers of anti-core antibodies contained high HCV RNA level. Uncertain results of anti-HCV in 15.2% of potential blood donors' samples were confirmed by detection of HCV RNA in low concentration. It proved OCI presence in these individuals and called for testing for wide pattern of HCV markers in addition to routine screening. Patients with hematological diseases showed low level of HCV RNA along with low titers of antibodies against one or two viral antigens. Competing Interests: The authors declare no conﬂict of interest.
Databáze:	MEDLINE
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