| Autor: |
Moskalev A; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.; Institute of Biology of Komi Science Center of Ural Branch of Russian Academy of Sciences, Syktyvkar, Russia.; Department of Biological and Medical Physics, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Guvatova Z; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia., Shaposhnikov M; Institute of Biology of Komi Science Center of Ural Branch of Russian Academy of Sciences, Syktyvkar, Russia., Lashmanova E; Department of Biological and Medical Physics, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Proshkina E; Institute of Biology of Komi Science Center of Ural Branch of Russian Academy of Sciences, Syktyvkar, Russia., Koval L; Institute of Biology of Komi Science Center of Ural Branch of Russian Academy of Sciences, Syktyvkar, Russia., Zhavoronkov A; Insilico Medicine, Baltimore, MD, United States., Krasnov G; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia., Kudryavtseva A; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. |
| Jazyk: |
angličtina |
| Zdroj: |
Frontiers in genetics [Front Genet] 2019 Mar 05; Vol. 10, pp. 149. Date of Electronic Publication: 2019 Mar 05 (Print Publication: 2019). |
| DOI: |
10.3389/fgene.2019.00149 |
| Abstrakt: |
Some effects of aging in animals are tissue-specific. In D. melanogaster neuronal overexpression of Gclc increases lifespan and improves certain physiological parameters associated with health benefits such as locomotor activity, circadian rhythmicity, and stress resistance. Our previous transcriptomic analyses of Drosophila heads, primarily composed of neuronal tissue, revealed significant changes in expression levels of genes involved in aging-related signaling pathways (Jak-STAT, MAPK, FOXO, Notch, mTOR, TGF-beta), translation, protein processing in endoplasmic reticulum, proteasomal degradation, glycolysis, oxidative phosphorylation, apoptosis, regulation of circadian rhythms, differentiation of neurons, synaptic plasticity, and transmission. Considering that various tissues age differently and age-related gene expression changes are tissue-specific, we investigated the effects of neuronal Gclc overexpression on gene expression levels in the imago thorax, which is primarily composed of muscles. A total of 58 genes were found to be differentially expressed between thoraces of control and Gclc overexpressing flies. The Gclc level demonstrated associations with expression of genes involved in the circadian rhythmicity, the genes in categories related to the muscle system process and the downregulation of genes involved in proteolysis. Most of the functional categories altered by Gclc overexpression related to metabolism including Drug metabolism, Metabolism of xenobiotics by cytochrome P450, Glutathione metabolism, Starch and sucrose metabolism, Citrate cycle (TCA cycle), One carbon pool by folate. Thus, the transcriptomic changes caused by neuron-specific Gclc overexpression in the thorax were less pronounced than in the head and affected pathways also differed from previous results. Although these pathways don't belong to the canonical longevity pathways, we suggest that they could participate in the delay of aging of Gclc overexpressing flies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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