Reduced CD27 - IgD - B Cells in Blood and Raised CD27 - IgD - B Cells in Gut-Associated Lymphoid Tissue in Inflammatory Bowel Disease.

Autor:	Pararasa C; Peter Gorer Department of Immunobiology, King's College London, Guy's Hospital, London, United Kingdom., Zhang N; Peter Gorer Department of Immunobiology, King's College London, Guy's Hospital, London, United Kingdom.; Obstetrics and Gynecology Hospital, Institutes of Biomedical Sciences (IBS), Fudan University, Shanghai, China., Tull TJ; Peter Gorer Department of Immunobiology, King's College London, Guy's Hospital, London, United Kingdom., Chong MHA; Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University London, London, United Kingdom., Siu JHY; Department of Surgery, Addenbrookes Hospital, University of Cambridge, Cambridge, United Kingdom., Guesdon W; Peter Gorer Department of Immunobiology, King's College London, Guy's Hospital, London, United Kingdom., Chavele KM; Peter Gorer Department of Immunobiology, King's College London, Guy's Hospital, London, United Kingdom., Sanderson JD; Department of Gastroenterology, Guy's and St Thomas' NHS Foundation Trust, London, United Kingdom., Langmead L; Department of Gastroenterology, Royal London Hospital, Barts Health, London, United Kingdom., Kok K; Department of Gastroenterology, Royal London Hospital, Barts Health, London, United Kingdom., Spencer J; Peter Gorer Department of Immunobiology, King's College London, Guy's Hospital, London, United Kingdom., Vossenkamper A; Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University London, London, United Kingdom.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2019 Mar 05; Vol. 10, pp. 361. Date of Electronic Publication: 2019 Mar 05 (Print Publication: 2019).
DOI:	10.3389/fimmu.2019.00361
Abstrakt:	The intestinal mucosa in inflammatory bowel disease (IBD) contains increased frequencies of lymphocytes and a disproportionate increase in plasma cells secreting immunoglobulin (Ig)G relative to other isotypes compared to healthy controls. Despite consistent evidence of B lineage cells in the mucosa in IBD, little is known of B cell recruitment to the gut in IBD. Here we analyzed B cells in blood of patients with Crohn's disease (CD) and ulcerative colitis (UC) with a range of disease activities. We analyzed the frequencies of known B cell subsets in blood and observed a consistent reduction in the proportion of CD27 - IgD - B cells expressing all Ig isotypes in the blood in IBD (independent of severity of disease and treatment) compared to healthy controls. Successful treatment of patients with biologic therapies did not change the profile of B cell subsets in blood. By mass cytometry we demonstrated that CD27 - IgD - B cells were proportionately enriched in the gut-associated lymphoid tissue (GALT) in IBD. Since production of TNFα is a feature of IBD relevant to therapies, we sought to determine whether B cells in GALT or the CD27 - IgD - subset in particular could contribute to pathology by secretion of TNFα or IL-10. We found that donor matched GALT and blood B cells are capable of producing TNFα as well as IL-10, but we saw no evidence that CD27 - IgD - B cells from blood expressed more TNFα compared to other subsets. The reduced proportion of CD27 - IgD - B cells in blood and the increased proportion in the gut implies that CD27 - IgD - B cells are recruited from the blood to the gut in IBD. CD27 - IgD - B cells have been implicated in immune responses to intestinal bacteria and recruitment to GALT, and may contribute to the intestinal inflammatory milieu in IBD.
Databáze:	MEDLINE
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