A nano sensor for sensitive and selective detection of Cu 2+ based on fluorescein: Cell imaging and drinking water analysis.

Autor: Mahajan PG; Department of Chemistry, Kongju National University, Gongju, Chungnam 32588, Republic of Korea., Dige NC; Department of Biological Sciences, Kongju National University, Gongju, Chungnam 32588, Republic of Korea., Vanjare BD; Department of Chemistry, Kongju National University, Gongju, Chungnam 32588, Republic of Korea., Eo SH; Department of Biological Sciences, Kongju National University, Gongju, Chungnam 32588, Republic of Korea., Kim SJ; Department of Biological Sciences, Kongju National University, Gongju, Chungnam 32588, Republic of Korea., Lee KH; Department of Chemistry, Kongju National University, Gongju, Chungnam 32588, Republic of Korea. Electronic address: khlee@kongju.ac.kr.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2019 Jun 05; Vol. 216, pp. 105-116. Date of Electronic Publication: 2019 Mar 11.
DOI: 10.1016/j.saa.2019.03.021
Abstrakt: A fluorescein-based nano probe was designed and synthesized for ultra-sensitive detection of Cu 2+ in aqueous solution. The formation of fluorescent organic nanoparticles confirmed by using particle size analysis and scanning electron microscopy. UV-Vis. absorption and fluorescence spectroscopy displays excellent photophysical properties of prepared nanoparticles as compared to parent molecule i.e. N-(3',6'-dihydroxy-3-oxo-3,3a-dihydrospiro[isoindole-1,9'-xanthene]-2(7aH)-yl)-1-naphthamide (FNH) in acetone. A series of 18 metal ion was examined with FNH nanoparticles (FNHNPs) to examine the change in fluorescence response. Pleasingly, only copper ion (Cu 2+ ) shows selective and sensitive fluorescence enhancement effect, which discussed on chelation-enhanced fluorescence phenomenon. Other competing metal ions does not affect the FNHNPs fluorescence enhancement induced by Cu 2+ ion. The excited state complexation through chelation-enhanced fluorescence of FNHNPs was further supported by UV-Vis. absorption and fluorescence decay titration of FNHNPs with and without the addition of Cu 2+ . The present investigation approach serves extremely low detection limit of 1.62 ng/mL (0.024 μM) for Cu 2+ in aqueous solution. In addition, benefit of present study includes practical application for the quantitative estimation of Cu 2+ in drinking water sample and intracellular cell imaging for Cu 2+ .
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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