Significance of CEP78 and WDR62 gene expressions in differentiated thyroid carcinoma: Possible predictors of lateral lymph node metastasis.

Autor: Hammad MO; Medical Biochemistry Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Elabbasy LM; Medical Biochemistry Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Abd Elghaffar MA; Mansoura Oncology Center, Mansoura University, Mansoura, Egypt., Zaki MMA; Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Bazeed FB; Medical Biochemistry Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Zahran MA; Medical Biochemistry Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Jazyk: angličtina
Zdroj: Asia-Pacific journal of clinical oncology [Asia Pac J Clin Oncol] 2019 Oct; Vol. 15 (5), pp. e154-e161. Date of Electronic Publication: 2019 Mar 18.
DOI: 10.1111/ajco.13143
Abstrakt: Objectives: This study aimed at investigating the clinical significance of CEP78 and WDR62 in differentiated thyroid carcinoma (DTC). This study also aimed at finding predictors that help in detecting patients with DTC who have high risk for lateral lymph node metastasis (LNM).
Methods: Quantitative real-time polymerase chain reaction (RT-qPCR) was performed to examine CEP78, and WDR62 mRNA expressions in 40 tissue specimens of DTC, and 40 goiter tissue specimens. Additionally, we reviewed clinical, ultrasound, laboratory, pathological data of patients to analyze the associations between these characteristics and lateral LNM.
Results: Our results demonstrated that relative CEP78 mRNA levels were significantly decreased in thyroid cancer tissues than goiter tissues (P = 0.002). ROC curve analysis confirmed the diagnostic value of CEP78 mRNA expression, providing an AUC equals to 0.698 (95% confidence intervals (CI), 0.583-0.813; P = 0.002). The relative WDR62 mRNA expression was not statistically different in DTC tissues and goiter tissues (P = 0.686). Furthermore, the DTC patients had been included to examine risk factors for lateral LNM. In multivariate analysis, the significant factors for predicting lateral LNM were low CEP78 mRNA expression (cut off value ≤0.54; P = 0.03; OR = 19.62; 95% CI, 1.3-296.23), central LNM (P = 0.011; OR = 33.6; 95% CI, 2.24-503.6) and calcifications (P = 0.023; OR = 27.187; 95% CI, 1.57-469.5).
Conclusions: CEP78 can be used as a promising molecular biomarker for differentiation between DTC and goiter tissues, in addition it might serve as a predictor of lateral LNM in DTC along with central LNM and calcifications. Unlike CEP78, WDR62 mRNA expression was not statistically different in DTC and goiter.
(© 2019 John Wiley & Sons Australia, Ltd.)
Databáze: MEDLINE
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