| Autor: |
Dragulska SA; Department of Chemistry, Brooklyn College, The City University of New York., Wlodarczyk MT; Department of Chemistry, Brooklyn College, The City University of New York; Ph.D. Program in Chemistry, The Graduate Center of The City University of New York., Poursharifi M; Department of Chemistry, Brooklyn College, The City University of New York; Ph.D. Program in Biochemistry, The Graduate Center of The City University of New York., Martignetti JA; Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai; Women's Health Research Institute, Icahn School of Medicine at Mount Sinai; Laboratory for Translational Research, Western Connecticut Health Network., Mieszawska AJ; Department of Chemistry, Brooklyn College, The City University of New York; Ph.D. Program in Chemistry, The Graduate Center of The City University of New York; Ph.D. Program in Biochemistry, The Graduate Center of The City University of New York; Aneta.Mieszawska@brooklyn.cuny.edu. |
| Abstrakt: |
We describe a method to produce a nanoemulsion composed of an oleic acids-Pt(II) core and a lysine-tyrosine-phenylalanine (KYF) coating (KYF-Pt-NE). The KYF-Pt-NE encapsulates Pt(II) at 10 wt. %, has a diameter of 107 ± 27 nm and a negative surface charge. The KYF-Pt-NE is stable in water and in serum, and is biologically active. The conjugation of a fluorophore to KYF allows the synthesis of a fluorescent nanoemulsion that is suitable for biological imaging. The synthesis of the nanoemulsion is performed in an aqueous environment, and the KYF-Pt-NE forms via self-assembly of a short KYF peptide and an oleic acids-platinum(II) conjugate. The self-assembly process depends on the temperature of the solution, the molar ratio of the substrates, and the flow rate of the substrate addition. Crucial steps include maintaining the optimal stirring rate during the synthesis, permitting sufficient time for self-assembly, and pre-concentrating the nanoemulsion gradually in a centrifugal concentrator. |
