Fish oil-based injectable lipid emulsions containing medium-chain triglycerides or added α-tocopherol offer anti-inflammatory benefits in a murine model of parenteral nutrition-induced liver injury.

Autor:	Baker MA; Vascular Biology Program and Department of Surgery., Cho BS; Vascular Biology Program and Department of Surgery., Anez-Bustillos L; Vascular Biology Program and Department of Surgery., Dao DT; Vascular Biology Program and Department of Surgery., Pan A; Vascular Biology Program and Department of Surgery., O'Loughlin AA; Vascular Biology Program and Department of Surgery., Lans ZM; Robert Wood Johnson Medical School, Piscataway, NJ., Mitchell PD; Institutional Centers for Clinical and Translational Research., Nosé V; Department of Pathology, Massachusetts General Hospital, Boston, MA., Gura KM; Department of Pharmacy, Boston Children's Hospital, Boston, MA., Puder M; Vascular Biology Program and Department of Surgery., Fell GL; Vascular Biology Program and Department of Surgery.
Jazyk:	angličtina
Zdroj:	The American journal of clinical nutrition [Am J Clin Nutr] 2019 Apr 01; Vol. 109 (4), pp. 1038-1050.
DOI:	10.1093/ajcn/nqy370
Abstrakt:	Background: Fish oil (FO) intravenous lipid emulsions (ILEs) are used as a monotherapy to treat parenteral nutrition (PN)-associated liver disease and provide essential fatty acids (EFAs) needed to sustain growth and prevent EFA deficiency (EFAD). Studies have suggested that medium-chain triglycerides (MCTs) and α-tocopherol have anti-inflammatory properties. Objective: The purpose of this study was to test whether FO-ILEs containing MCTs and/or additional α-tocopherol decrease the inflammatory response to an endotoxin challenge compared with FO-ILE alone and preserve the ability to prevent PN-induced liver injury in mice. Methods: A murine model of PN-induced hepatosteatosis was used to compare the effects of ILEs formulated in the laboratory containing varying ratios of FO and MCTs, and subsequently FO- and 50:50 FO:MCT-ILE plus 500 mg/L α-tocopherol (FO + AT and 50:50 + AT, respectively). C57BL/6 mice receiving unpurified diet (UPD), PN-equivalent diet (PN) + saline, and PN + soybean oil (SO)-ILE served as controls. After 19 d, mice received an intraperitoneal saline or endotoxin challenge 4 h before being killed. Serum and livers were harvested for histologic analysis, fatty acid profiling, and measurement of systemic inflammatory markers (tumor necrosis factor-α, interleukin-6). Results: All ILEs were well tolerated and prevented biochemical EFAD. Livers of mice that received saline and SO developed steatosis. Mice that received 30:70 FO:MCT developed mild hepatosteatosis. All other FO-containing ILEs preserved normal hepatic architecture. Mice that received FO- or SO-ILE had significantly elevated systemic inflammatory markers after endotoxin challenge compared with UPD-fed controls, whereas 50:50 FO:MCT, 30:70 FO:MCT, FO + AT, and 50:50 + AT groups had significantly lower inflammatory markers similar to those seen in UPD-fed controls. Conclusions: Mixed FO/MCT and the addition of α-tocopherol to FO improved the inflammatory response to endotoxin challenge compared with FO-ILE alone while still preventing PN-induced liver injury and EFAD in mice. There was no synergistic relation between α-tocopherol and MCTs. (Copyright © American Society for Nutrition 2019.)
Databáze:	MEDLINE
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