Divergent coronaviruses detected in wild birds in Brazil, including a central park in São Paulo.

Autor: Barbosa CM; Institute of Biomedical Sciences, University of Sao Paulo, 1374 Prof. Lineu Prestes avenue, São Paulo, 05508-900, Brazil., Durigon EL; Institute of Biomedical Sciences, University of Sao Paulo, 1374 Prof. Lineu Prestes avenue, São Paulo, 05508-900, Brazil., Thomazelli LM; Institute of Biomedical Sciences, University of Sao Paulo, 1374 Prof. Lineu Prestes avenue, São Paulo, 05508-900, Brazil., Ometto T; Institute of Biomedical Sciences, University of Sao Paulo, 1374 Prof. Lineu Prestes avenue, São Paulo, 05508-900, Brazil., Marcatti R; Wildlife and Green Areas Department, São Paulo Municipality, São Paulo, Brazil., Nardi MS; Wildlife and Green Areas Department, São Paulo Municipality, São Paulo, Brazil., de Aguiar DM; Laboratório de Virologia e Rickettsioses, Hospital Veterinário, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil., Pinho JB; Laboratório de Ecologia de Aves, Instituto de Biociências, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil., Petry MV; Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, Rio Grande do Sul, Brazil., Neto IS; Instituto Chico Mendes de Conservação da Biodiversidade (ICMBio), Brasília, Brazil., Serafini P; Centro Nacional de Pesquisa e Conservação de Aves Silvestres (CEMAVE), Cabedelo, Paraíba, Brazil., Rodrigues RC; Universidade Federal da Paraíba (UFPB), João Pessoa, Paraíba, Brazil., de Azevedo Junior SM; Universidade Federal Rural de Pernambuco (UFRPE), Recife, Pernambuco, Brazil., Góes LGB; Institute of Biomedical Sciences, University of Sao Paulo, 1374 Prof. Lineu Prestes avenue, São Paulo, 05508-900, Brazil., de Araujo J; Institute of Biomedical Sciences, University of Sao Paulo, 1374 Prof. Lineu Prestes avenue, São Paulo, 05508-900, Brazil. jansentequila@usp.br.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2019 Apr; Vol. 50 (2), pp. 547-556. Date of Electronic Publication: 2019 Mar 15.
DOI: 10.1007/s42770-019-00065-7
Abstrakt: Coronaviruses are single-stranded positive-sense RNA viruses associated with important avian diseases. Their relatively high rates of mutation and recombination frequencies allow them to adapt to new hosts and ecological niches. Although Brazil has 18% of global avian species diversity, studies regarding the presence of avian viral diseases in wild birds in South America are scarce. In this study, we performed a retrospective analysis of the presence of CoVs in 746 wild birds. Oropharyngeal and cloacal swabs were obtained and placed together in vials containing VTM transport medium collected in different regions of Brazil between 2006 and 2013. Screening for viral nucleic acid was performed using conventional RT-PCR and pancoronavirus nested PCR. Positive samples were characterized by partial sequencing of the RNA-dependent RNA polymerase (RdRp) gene, and ensuing phylogenetic analysis was performed to investigate the association between virus epidemiology and bird migration routes. Coronavirus RNA were detected and sequenced from six samples, in which three were related to gammacoronaviruses group and the other three to deltacoronavirus group. Our study documents the presence of CoVs related to avian gamma- and deltacoronaviruses circulating in both urban- and poultry-farm regions of Brazil, implicating wild birds as potential carriers of CoVs which may represent a risk to poultry farms and public health in Brazil.
Databáze: MEDLINE
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