Antimicrobial activity of Phyllanthus amarus Schumach. & Thonn and inhibition of the NorA efflux pump of Staphylococcus aureus by Phyllanthin.

Autor: Braga Ribeiro AM; Medicinal Plants Research Center, Federal University of Piaui, Teresina, Piaui, Brazil., Sousa JN; Laboratory of Research in Microbiology, Federal University of Piaui, Teresina, Piaui, Brazil., Costa LM; Laboratory of Research in Microbiology, Federal University of Piaui, Teresina, Piaui, Brazil., Oliveira FAA; Laboratory of Research in Microbiology, Federal University of Piaui, Teresina, Piaui, Brazil., Dos Santos RC; Nucleus of Research in Biodiversity and Biotechnology, Biotec, Federal University of Piaui, Parnaiba, Piaui, Brazil., Silva Nunes AS; Medicinal Plants Research Center, Federal University of Piaui, Teresina, Piaui, Brazil., da Silva WO; Institute of Chemistry of São Carlos, University of São Paulo, São Paulo, Brazil., Marques Cordeiro PJ; Institute of Chemistry of São Carlos, University of São Paulo, São Paulo, Brazil., de Sousa Lima Neto J; Laboratory of Organic Geochemistry, Federal University of Piaui, Teresina, Piauí, Brazil., de Siqueira-Júnior JP; Laboratory of Genetics of Microorganisms, Federal University of Paraiba, Joao Pessoa, Paraiba, Brazil., Kaatz GW; Department of Medicine, Division of Infectious Diseases, Wayne State University School of Medicine, Detroit, MI, USA., Barreto HM; Laboratory of Research in Microbiology, Federal University of Piaui, Teresina, Piaui, Brazil. Electronic address: hmbarreto@ufpi.edu.br., de Oliveira AP; Medicinal Plants Research Center, Federal University of Piaui, Teresina, Piaui, Brazil.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2019 May; Vol. 130, pp. 242-246. Date of Electronic Publication: 2019 Mar 12.
DOI: 10.1016/j.micpath.2019.03.012
Abstrakt: The aim of this study was to evaluate the antimicrobial activity of ethanoic extract of P. amarus (PAEE) and its compound Phyllanthin, as well as, investigate if these natural products could modulate the fluoroquinolone-resistance in S. aureus SA1199-B by way of overexpression of the NorA efflux pump. Microdilution tests were carried out to determine the minimal inhibitory concentration (MIC) of the PAEE or Phyllanthin against several bacterial and yeast strains. To evaluate if PAEE or Phyllanthin were able to act as modulators of the fluoroquinolone-resistance, MICs for Norfloxacin and ethidium bromide were determined in the presence or absence of PAEE or Phyllanthin against S. aureus SA1199-B. PAEE showed antimicrobial activity against Gram-negative strains, meanwhile Phyllanthin was inactive against all strains tested. Addition of PAEE or Phyllanthin, to the growth media at sub-inhibitory concentrations enhanced the activity of the Norfloxacin as well as, Ethidium Bromide, against S. aureus SA1199-B. These results indicate that Phyllanthin is able to modulate the fluoroquinolone-resistance possibly by inhibition of NorA. This hypothesis was supported by in silico docking analysis which confirmed that Phyllantin is a NorA ligand. Thus, this compound could be used as a potentiating agent of the Norfloxacin activity in the treatment of infections caused by fluoroquinolone-resistant S. aureus.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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