Acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection.

Autor: Mazlan NF; Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia. Electronic address: fadysagiprincess9@gmail.com., Tan LL; Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia. Electronic address: lingling@ukm.edu.my., Karim NHA; School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia. Electronic address: nurulhuda@ukm.edu.my., Heng LY; Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia; School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia. Electronic address: leeyokheng@yahoo.co.uk., Jamaluddin ND; Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia. Electronic address: diyanajamaluddin@gmail.com., Yusof NYM; School of Biosciences & Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia. Electronic address: yuziana@ukm.edu.my., Quay DHX; School of Biosciences & Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia. Electronic address: dorisquay@ukm.edu.my., Khalid B; Department of Medicine, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia; Hospital Serdang, Jalan Puchong, 43000 Kajang, Selangor Darul Ehsan, Malaysia. Electronic address: bahariah@upm.edu.my.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2019 Jun 01; Vol. 198, pp. 358-370. Date of Electronic Publication: 2019 Feb 06.
DOI: 10.1016/j.talanta.2019.02.036
Abstrakt: An optical genosensor based on Schiff base complex (Zn 2+ salphen) DNA label and acrylic microspheres (AMs) as polymer support of the capturing DNA probe (cpDNA) was developed for dengue virus serotype 2 (DEN-2) detection via reflectance spectrophotometric method. The solid-state optical DNA biosensor showed high selectivity and specificity up to one-base mismatch in the target DNA sequence owing to the salphen chemical structure that is rich in localized electrons, and allowed π-π stacking interaction between stacked base pairs of double-stranded DNA (dsDNA). The reflectometric DNA microsensor demonstrated a broad linear detection range towards DEN-2 DNA from 1 × 10 -15 M to 1 × 10 -3 M with a low limit of detection (LOD) obtained at 1.21 × 10 -16 M. The DNA biosensor gave reproducible optical response with a satisfactory relative standard deviation (RSD) at 3.1%, (n = 3), and the reflectance response was stable even after four regeneration cycles of the DNA biosensor. The optical genosensor was proven comparable with standard reverse transcription polymerase chain reaction (RT-PCR) in detecting DEN-2 genome acquired from clinical samples of serum, urine and saliva of dengue virus infected patients under informed consent. The developed reflectometric DNA biosensor is advantageous in offering an early DEN-2 diagnosis, when fever symptom started to manifest in patient.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE