Bioactive 2-(Methyldithio)Pyridine-3-Carbonitrile from Persian Shallot ( Allium stipitatum Regel.) Exerts Broad-Spectrum Antimicrobial Activity.

Autor: Karunanidhi A; Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia. arunmscimb@gmail.com.; Department of Pharmacology and Chemistry, Faculty of Pharmacy, Universiti Teknologi MARA, Bandar Puncak Alam 42300, Selangor Darul Ehsan, Malaysia. arunmscimb@gmail.com., Ghaznavi-Rad E; Department of Microbiology and Immunology, Faculty of Medicine, Arak University of Medical Sciences, Basij Square, Arak 38481-7-6941, Iran. ghaznaviehs@yahoo.com., Jeevajothi Nathan J; School of Medicine, Taylor's University, Lakeside Campus, No. 1, Jalan Taylor's, Subang Jaya 47500, Selangor Darul Ehsan, Malaysia. jjayakayatri@yahoo.com., Joseph N; Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia. narcissemsjoseph@gmail.com., Chigurupati S; Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraidah 52571, Kingdom of Saudi Arabia. sridevi.phd@gmail.com., Mohd Fauzi F; Department of Pharmacology and Chemistry, Faculty of Pharmacy, Universiti Teknologi MARA, Bandar Puncak Alam 42300, Selangor Darul Ehsan, Malaysia. fazlin205@gmail.com., Pichika MR; Department of Pharmaceutical Chemistry, School of Pharmacy, International Medical University, 126 Jalan Bukit Jalil, Kuala Lumpur 57000, Malaysia. mallikarjunarao_pichika@imu.edu.my., Hamat RA; Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia. rukman@upm.edu.my., Lung LTT; Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia. leslie@upm.edu.my., van Belkum A; R & D Microbiology, BioMerieux, 3 route de Port Michaud, 38390 La Balme-les-Grottes, France. alex.vanbelkum@biomerieux.com., Neela V; Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia. vasantha@upm.edu.my.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2019 Mar 13; Vol. 24 (6). Date of Electronic Publication: 2019 Mar 13.
DOI: 10.3390/molecules24061003
Abstrakt: Antibiotic resistance is a problem that continues to challenge the healthcare sector, especially in clinically significant pathogens like methicillin-resistant Staphylococcus aureus (MRSA). Herein is described the isolation and structure elucidation of a bioactive compound from Allium stipitatum with antimicrobial activity. Crude Allium stipitatum dichloromethane extract (ASDE) was subjected to systematic purification by chromatographic procedures to afford various bioactive fractions. A fraction that exhibited anti-MRSA activity (4 µg·mL -1 ) was further characterized to determine the structure. The structure of the compound was elucidated as 2-(methyldithio)pyridine-3-carbonitrile (2-Medpy-3-CN). The 2-Medpy-3-CN compound, which was screened for antimicrobial activity, exhibited minimum inhibitory concentrations (MICs) in the range of 0.5 to >64 µg·mL -1 for tested bacterial species and 0.25 to 2 µg·mL -1 for Candida spp. Further studies are important to confirm the drug target and mechanism of action.
Databáze: MEDLINE
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