Alterations in monocyte subsets and cytokine production after TLR activation in American Cutaneous Leishmaniasis.

Autor: Quixabeira VBL; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal Goiás, Goiânia, Goiás, Brazil.; Instituto Goiano de Oncologia e Hematologia, Goiânia, Goiás, Brazil., Pereira LIA; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal Goiás, Goiânia, Goiás, Brazil.; Hospital AnuarAuad (Doenças Tropicais), Goiânia, Goiás, Brazil., Veras PRV; Centro Universitário Unirg, Gurupi, Tocantins, Goiás, Brazil., da Costa ACV; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal Goiás, Goiânia, Goiás, Brazil., Fonseca LG; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal Goiás, Goiânia, Goiás, Brazil., Galdino H Jr; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal Goiás, Goiânia, Goiás, Brazil., da Silva IA Jr; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal Goiás, Goiânia, Goiás, Brazil., Morato CI; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal Goiás, Goiânia, Goiás, Brazil., Pinto SA; Instituto Goiano de Oncologia e Hematologia, Goiânia, Goiás, Brazil.; Faculdade de Medicina, Universidade Federal de Goiás, Goiânia, Goiás, Brazil., Pereira AJCS; Hospital AnuarAuad (Doenças Tropicais), Goiânia, Goiás, Brazil., Dorta ML; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal Goiás, Goiânia, Goiás, Brazil., Oliveira MAP; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal Goiás, Goiânia, Goiás, Brazil., Gomes RS; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal Goiás, Goiânia, Goiás, Brazil., Ribeiro-Dias F; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal Goiás, Goiânia, Goiás, Brazil.
Jazyk: angličtina
Zdroj: Parasite immunology [Parasite Immunol] 2019 May; Vol. 41 (5), pp. e12623. Date of Electronic Publication: 2019 Apr 10.
DOI: 10.1111/pim.12623
Abstrakt: Phenotypic and functional aspects of monocytes from Localized Cutaneous Leishmaniasis (LCL) patients were evaluated. The frequencies of monocyte subsets and TLR2/TLR4 expression were evaluated in fresh peripheral blood whereas cytokine production was evaluated in whole blood cell cultures stimulated with TLR agonists or Leishmania braziliensis antigen (Ag). CD16 + monocytes frequency was increased in patients compared with controls. A TLR4 agonist (LPS) induced expression of TNF and IL-10 in monocyte subsets of patients and controls. The CD14 + CD16 + monocytes expressed higher levels of these cytokines than CD14 + CD16 - cells. The levels of secreted TNF were higher in whole blood cell cultures from patients than controls after LPS/TLR4 or Ag stimulation. Whereas in controls there was a positive correlation between TNF and IL-10 levels, this was not observed in stimulated cell cultures from patients. The high levels of LPS-induced TNF were associated with the number of lesions and the percentages of CD14 hi CD16 + monocytes. The levels of TLR2-induced TNF were also associated with number of lesions. All monocyte subsets from patients expressed higher levels of TLR2 and TLR4 than controls. Data suggest that systemically activated monocytes contribute for an imbalance in pro- and anti-inflammatory cytokine production during LCL, participating in the immunopathogenesis of the disease.
(© 2019 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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