Automated smartphone-based system for measuring sperm viability, DNA fragmentation, and hyaluronic binding assay score.

Autor: Dimitriadis I; Department of Obstetrics & Gynecology, Division of Reproductive Endocrinology and Infertility, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America., L Bormann C; Department of Obstetrics & Gynecology, Division of Reproductive Endocrinology and Infertility, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America., Kanakasabapathy MK; Department of Medicine, Division of Engineering in Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America., Thirumalaraju P; Department of Medicine, Division of Engineering in Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America., Kandula H; Department of Medicine, Division of Engineering in Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America., Yogesh V; Department of Medicine, Division of Engineering in Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America., Gudipati N; Department of Medicine, Division of Engineering in Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America., Natarajan V; Department of Medicine, Division of Engineering in Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America., C Petrozza J; Department of Obstetrics & Gynecology, Division of Reproductive Endocrinology and Infertility, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America., Shafiee H; Department of Medicine, Division of Engineering in Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America.; Department of Medicine, Harvard Medical School, Boston, Massachusetts, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2019 Mar 13; Vol. 14 (3), pp. e0212562. Date of Electronic Publication: 2019 Mar 13 (Print Publication: 2019).
DOI: 10.1371/journal.pone.0212562
Abstrakt: The fundamental test for male infertility, semen analysis, is mostly a manually performed subjective and time-consuming process and the use of automated systems has been cost prohibitive. We have previously developed an inexpensive smartphone-based system for at-home male infertility screening through automatic and rapid measurement of sperm concentration and motility. Here, we assessed the feasibility of using a similar smartphone-based system for laboratory use in measuring: a) Hyaluronan Binding Assay (HBA) score, a quantitative score describing the sperm maturity and fertilization potential in a semen sample, b) sperm viability, which assesses sperm membrane integrity, and c) sperm DNA fragmentation that assesses the degree of DNA damage. There was good correlation between the manual analysis and smartphone-based analysis for the HBA score when the device was tested with 31 fresh, unprocessed human semen samples. The smartphone-based approach performed with an accuracy of 87% in sperm classification when the HBA score was set at manufacturer's threshold of 80. Similarly, the sperm viability and DNA fragmentation tests were also shown to be compatible with the smartphone-based system when tested with 102 and 47 human semen samples, respectively.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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