Leaf heteroblasty in Passiflora edulis as revealed by metabolic profiling and expression analyses of the microRNAs miR156 and miR172.

Autor: Silva PO; Departamento de Biologia Vegetal/Instituto de Biotecnologia Aplicada a Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Batista DS; Departamento de Biologia Vegetal/Instituto de Biotecnologia Aplicada a Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.; Universidade Estadual do Maranhão, São Luís, MA, Brazil., Cavalcanti JHF; Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.; Instituto de Educação, Agricultura e Ambiente, Universidade Federal do Amazonas, Humaitá, Amazonas, Brazil., Koehler AD; Departamento de Biologia Vegetal/Instituto de Biotecnologia Aplicada a Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Vieira LM; Departamento de Biologia Vegetal/Instituto de Biotecnologia Aplicada a Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Fernandes AM; Departamento de Biologia Vegetal/Instituto de Biotecnologia Aplicada a Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Barrera-Rojas CH; Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, São Paulo, Brazil.; Instituto de Biociências, Universidade Estadual de São Paulo, Botucatu, São Paulo, Brazil., Ribeiro DM; Universidade Estadual do Maranhão, São Luís, MA, Brazil., Nogueira FTS; Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, São Paulo, Brazil., Otoni WC; Departamento de Biologia Vegetal/Instituto de Biotecnologia Aplicada a Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Jazyk: angličtina
Zdroj: Annals of botany [Ann Bot] 2019 Jul 08; Vol. 123 (7), pp. 1191-1203.
DOI: 10.1093/aob/mcz025
Abstrakt: Background and Aims: Juvenile-to-adult phase transition is marked by changes in leaf morphology, mostly due to the temporal development of the shoot apical meristem, a phenomenon known as heteroblasty. Sugars and microRNA-controlled modules are components of the heteroblastic process in Arabidopsis thaliana leaves. However, our understanding about their roles during phase-changing in other species, such as Passiflora edulis, remains limited. Unlike Arabidopsis, P. edulis (a semi-woody perennial climbing vine) undergoes remarkable changes in leaf morphology throughout juvenile-to-adult transition. Nonetheless, the underlying molecular mechanisms are unknown.
Methods: Here we evaluated the molecular mechanisms underlying the heteroblastic process by analysing the temporal expression of microRNAs and targets in leaves as well as the leaf metabolome during P. edulis development.
Key Results: Metabolic profiling revealed a unique composition of metabolites associated with leaf heteroblasty. Increasing levels of glucose and α-trehalose were observed during juvenile-to-adult phase transition. Accumulation of microRNA156 (miR156) correlated with juvenile leaf traits, whilst miR172 transcript accumulation was associated with leaf adult traits. Importantly, glucose may mediate adult leaf characteristics during de novo shoot organogenesis by modulating miR156-targeted PeSPL9 expression levels at early stages of shoot development.
Conclusions: Altogether, our results suggest that specific sugars may act as co-regulators, along with two microRNAs, leading to leaf morphological modifications throughout juvenile-to-adult phase transition in P. edulis.
(© The Author(s) 2019. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze: MEDLINE
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