Magnetic 3D scaffold: A theranostic tool for tissue regeneration and non-invasive imaging in vivo.

Autor: Sajesh KM; Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India., Ashokan A; Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India., Gowd GS; Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India., Sivanarayanan TB; Central animal lab facility, Amrita Institute of Medical sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India., Unni AKK; Central animal lab facility, Amrita Institute of Medical sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India., Nair SV; Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India. Electronic address: shantinair@aims.amrita.edu., Koyakutty M; Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India. Electronic address: manzoork@aims.amrita.edu.
Jazyk: angličtina
Zdroj: Nanomedicine : nanotechnology, biology, and medicine [Nanomedicine] 2019 Jun; Vol. 18, pp. 179-188. Date of Electronic Publication: 2019 Mar 08.
DOI: 10.1016/j.nano.2019.02.022
Abstrakt: We report an osteoconducting magnetic 3D scaffold using Fe 2+ doped nano-hydroxyapatite-Alginate-Gelatin (AGHFe1) for Magnetic Resonance Imaging based non-invasive monitoring of bone tissue regeneration. In rat cranial defect model, the scaffold facilitated non-invasive monitoring of cell migration, inflammatory response and matrix deposition by unique changes in transverse relaxation time (T2). Cell infiltration resulted in a considerable increase in T2 from ~37 to ~62 ms, which gradually returned to that of native bone (~23 ms) by 90 days. We used this method to compare in vivo performance of scaffold with bone-morphogenic protein-2 (AGHFe2) or faster degrading (AGHFe3). MRI and histological analysis over 90 days showed non-uniform bone formation in AGHFe1 with ∆T2 (T2 Native bone - T2 Regenerated bone ) ~13 ms, whereas, AGHFe2 and AGHFe3 showed ∆T2 ~ 09 and 05 ms respectively, suggesting better bone formation in AGHFe3. Thus, we show that MR-contrast enabled scaffold can help better assessment of bone-regeneration non-invasively.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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