Characterization of three glyphosate resistant Parthenium hysterophorus populations collected in citrus groves from Mexico.

Autor: Palma-Bautista C; Department of Agricultural Chemistry and Edaphology, University of Cordoba, 14071 Cordoba, Spain., Gherekhloo J; Department of Agronomy, Gorgan University of Agricultural Sciences and Natural Resources, 49189-43464 Gorgan, Iran. Electronic address: gherekhloo@yahoo.com., Domínguez-Martínez PA; National Institute of Forestry, Agriculture and Livestock Research (INIFAP)-Valle del Guadiana Experimental Field, 34170 Durango, Mexico., Domínguez-Valenzuela JA; Department of Agricultural Parasitology, Chapingo Autonomous University, 56230 Texcoco, Mexico., Cruz-Hipolito HE; Bayer CropScience Mexico, 11520 Mexico City, Mexico., Alcántara-de la Cruz R; Departamento de Quimica, Universidade Federal de São Carlos, 13565-905 São Carlos, Brazil., Rojano-Delgado AM; Department of Agricultural Chemistry and Edaphology, University of Cordoba, 14071 Cordoba, Spain., De Prado R; Department of Agricultural Chemistry and Edaphology, University of Cordoba, 14071 Cordoba, Spain.
Jazyk: angličtina
Zdroj: Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2019 Mar; Vol. 155, pp. 1-7. Date of Electronic Publication: 2018 Nov 03.
DOI: 10.1016/j.pestbp.2018.11.002
Abstrakt: Continuous use of glyphosate in citrus groves in the Gulf of Mexico region has selected for resistant Parthenium hysterophorus L. populations. In this study, the target-site and non-target-site resistance mechanisms were characterized in three putative glyphosate-resistant (GR) P. hysterophorus populations, collected in citrus groves from Acateno, Puebla (GR1 and GR2) and Martínez de la Torre, Veracruz (GR3), and compared with a susceptible population (GS). Based on plant mortality, the GR populations were 9.2-17.3 times more resistant to glyphosate than the GS population. The low shikimate accumulation in the GR population confirmed this resistance. Based on plant mortality and shikimate accumulation, the GR3 population showed intermediate resistance to glyphosate. The GR populations absorbed 15-28% less 14 C-glyphosate than the GS population (78.7% absorbed from the applied) and retained 48.7-70.7% of 14 C-glyphosate in the treated leaf, while the GS population translocated ~68% of absorbed herbicide to shoots and roots. The GR3 population showed the lowest translocation and absorption rates, but was found to be susceptible at the target site level. The 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene sequence of the GR1 and GR2 populations showed the Pro106-Ser mutation, conferring 19- and 25-times more resistance in comparison to the GS population, respectively. Reduced absorption and impaired translocation conferred glyphosate resistance on the GR3 population, and contributed partially to the resistance of the GR1 and GR2 populations. Additionally, the Pro-106-Ser mutation increased the glyphosate resistance of the last two P. hysterophorus populations.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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