Antiproliferative activity of ionic liquid-graviola fruit extract against human breast cancer (MCF-7) cell lines using flow cytometry techniques.

Autor: Daddiouaissa D; Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University, Malaysia (IIUM), P. O. Box 10, Gombak, 50728, Kuala Lumpur, Malaysia., Amid A; International Institute for Halal Research and Training (INHART), Level 3, KICT Building, International Islamic University Malaysia (IIUM), Jalan Gombak, 53100, Kuala Lumpur, Malaysia. Electronic address: azuraamid@iium.edu.my., Kabbashi NA; Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University, Malaysia (IIUM), P. O. Box 10, Gombak, 50728, Kuala Lumpur, Malaysia., Fuad FAA; Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University, Malaysia (IIUM), P. O. Box 10, Gombak, 50728, Kuala Lumpur, Malaysia., Elnour AM; Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University, Malaysia (IIUM), P. O. Box 10, Gombak, 50728, Kuala Lumpur, Malaysia., Epandy MAKMS; Adikafirdaus Resources, Lot 24, Jalan Klebang Selatan, 2/5 Kampung Tersusun, Batu 6 Klebang Selatan, 31200, Ipoh, Perak, Malaysia.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2019 May 23; Vol. 236, pp. 466-473. Date of Electronic Publication: 2019 Mar 07.
DOI: 10.1016/j.jep.2019.03.003
Abstrakt: Ethnopharmacological Relevance: Medicinal plants have been used for ages by indigenous communities around the world to help humankind sustain its health. Graviola (Annona muricata), also called soursop, is a member of the Annonaceae family and is an evergreen plant that is generally distributed in tropical and subtropical areas of the world. Graviola tree has a long history of traditional use due to its therapeutic potential including anti-inflammatory, antimicrobial, antioxidant, insecticide and cytotoxic to tumor cells.
Aim of the Study: This study aimed to investigate the in vitro antiproliferative effects and apoptotic events of the ionic liquid extract of Graviola fruit (IL-GFE) on MCF-7 breast cancer cells and their cytokinetics behaviour to observe their potential as a therapeutic alternative in cancer treatment.
Materials and Methods: The cell viability assay of the extract was measured using tetrazolium bromide (MTT assay) to observe the effects of Graviola fruit extract. Then the cytokinetics behaviour of MCF-7 cells treated with IL-GFE is observed by plotting the growth curve of the cells. Additionally, the cell cycle distribution and apoptosis mechanism of IL-GFE action on MCF-7 cancer cells were observed by flow cytometry.
Results: IL-GFE exhibited anti-proliferative activity on MCF-7 with the IC50 value of 4.75 μg/mL, compared to Taxol with an IC50 value of 0.99 μg/mL. IL- GFE also reduced the number of cell generations from 3.71 to 1.67 generations compared to 2.18 generations when treated with Taxol. Furthermore, the anti-proliferative activities were verified when the growth rate was decreased dynamically from 0.0077 h to 1 to 0.0035 h-1. Observation of the IL-GFE-treated MCF-7 under microscope demonstrated detachment of cells and loss of density. The growth inhibition of the cells by extracts was associated with cell cycle arrest at the G0/G1 phase, and phosphatidylserine externalisation confirms the anti-proliferation through apoptosis.
Conclusions: ionic liquid Graviola fruit extract affect the cytokinetics behaviour of MCF-7 cells by reducing cell viability, induce apoptosis and cell cycle arrest at the G0/G1 phase.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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