| Autor: |
Cavicchioli VQ; Departamento de Veterinária, Universidade Federal de Viçosa, Campus UFV, Viçosa, MG, 36570-900, Brazil., Todorov SD; Departamento de Veterinária, Universidade Federal de Viçosa, Campus UFV, Viçosa, MG, 36570-900, Brazil.; Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 580, São Paulo, SP, 05508-000, Brazil., Iliev I; Department of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University, 4 Tzar Asen Str, 4000, Plovdiv, Bulgaria., Ivanova I; Department of General and Applied Microbiology, Faculty of Biology, Sofia University 'St. Kliment Ohridski', 8 Dragan Tzankov Blvd., 1164, Sofia, Bulgaria., Drider D; Université de Lille, INRA, Université d'Artois, Université du Littoral-Côte d'Opale, EA 7394-ICV-Institut Charles Viollette, F-59000, Lille, France., Nero LA; Departamento de Veterinária, Universidade Federal de Viçosa, Campus UFV, Viçosa, MG, 36570-900, Brazil. nero@ufv.br. |
| Abstrakt: |
The bacteriocinogenic Enterococcus hirae ST57ACC recently isolated from a Brazilian artisanal cheese was subjected here to additional analyses in order to evaluate its bacteriocin production and the potential influence of ABC transporter system in its expression. Besides these physiological and molecular aspects, the bacteriocin was evaluated for its cytotoxicity against HT-29. Differences in the inoculum size had no impact on the growth of E. hirae ST57ACC; however, the bacteriocin was only produced after 9 h of growth when the strain was inoculated at 5% or 10% (v/v), with similar levels of bacteriocin production obtained by both conventional growth and batch fermentation. Furthermore, potential expression of ABC transporters corresponding to the bacteriocin transport and sugar metabolism was identified. In terms of adverse effects, when a semi-purified fraction of the bacteriocin and the cell-free supernatant were tested against HT-29, total cell viability was similar to observed on untreated cells, indicating the absence of cytotoxic effect. Based on the obtained results, E. hirae ST57ACC can produce its bacteriocin at industrial level by using bioreactors, its bacteriocin expression is potentially influenced by the ABC transporter system, and no cytotoxic effects were observed on HT-29 cells, indicating its potential use as a bio-preservative. |
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