Genotypic and phenotypic characterization of Edwardsiella isolates from different fish species and geographical areas in Asia: Implications for vaccine development.

Autor: Dubey S; Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway., Maiti B; UNESCO MIRCEN for Medical and Marine Biotechnology, Nitte University Centre for Science Education and Research, NITTE (Deemed to be University), Mangalore, India., Kim SH; Fishcare Co. Ltd., Seogwipo-si, Korea., Sivadasan SM; Department of Fisheries Microbiology, College of Fisheries, Karnataka Veterinary Animal & Fisheries Sciences University, Mangalore, India., Kannimuthu D; ICAR- Central Institute of Fisheries Education, Mumbai, India., Pandey PK; ICAR- Central Institute of Fisheries Education, Mumbai, India., Girisha SK; Department of Fisheries Microbiology, College of Fisheries, Karnataka Veterinary Animal & Fisheries Sciences University, Mangalore, India., Mutoloki S; Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway., Chen SC; Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan., Evensen Ø; Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway., Karunasagar I; UNESCO MIRCEN for Medical and Marine Biotechnology, Nitte University Centre for Science Education and Research, NITTE (Deemed to be University), Mangalore, India., Munang'andu HM; Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway.
Jazyk: angličtina
Zdroj: Journal of fish diseases [J Fish Dis] 2019 Jun; Vol. 42 (6), pp. 835-850. Date of Electronic Publication: 2019 Mar 08.
DOI: 10.1111/jfd.12984
Abstrakt: The genus Edwardsiella is one of the major causes of fish diseases globally. Herein, we examined 37 isolates from ten different fish species from India, South Korea and Taiwan to gain insight into their phenotypic and genotypic properties, of which 30 were characterized as E. tarda with phenotypic homology estimated at 85.71% based on API-20E biochemical tests. Genotyping using 16S rRNA put all isolates together with E. anguillarum, E. hoshinae, E. tarda, E. piscicida and E. ictaluri reference strains in a monophyletic group. In contrast, the gyrB phylogenetic tree clearly separated E. ictaluri, E. tarda and E. hoshinae reference strains from our isolates and put our isolates into two groups with group I being homologous with the E. anguillarum reference strain while group II was homologous with the E. piscicida reference strain. Hence, our findings point to E. piscicida and E. anguillarum as species infecting different fish species in Asia. Homology of the ompW protein suggested that strains with broad protective coverage could be identified as vaccine candidates. This study underscores the importance of combining genotyping with phenotyping for valid species classification. In addition, it accentuates the importance of phylogenetic comparison of bacterial antigens for identification of potential vaccine candidates.
(© 2019 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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