Signaling roadmap to epithelial-mesenchymal transition in pterygium, TWIST1 centralized.

Autor: Meshkani SE; Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran., Kooshan N; Department of Genetics and Biotechnology, School of Biological Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran., Moghadam AB; Sabzevar Branch, Islamic Azad University, Sabzevar, Iran., Falanji F; Department of Physiology, School of Paramedical Sciences, Neuroscience Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran., Adli A; Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran., Baghbani-Arani F; Department of Genetics and Biotechnology, School of Biological Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran., Arian AG; Arian Hospital, Sabzevar University of Medical Sciences, Sabzevar, Iran., Rad A; Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran.
Jazyk: angličtina
Zdroj: Journal of cellular physiology [J Cell Physiol] 2019 Aug; Vol. 234 (10), pp. 18146-18155. Date of Electronic Publication: 2019 Mar 07.
DOI: 10.1002/jcp.28447
Abstrakt: Pterygium as a complex disease shares common features with other malignant cells in its onset recurrence and especially epithelial-mesenchymal transition (EMT) transition. Although using different approaches including conjunctival autografts, amniotic membrane, radiotherapy, mitomycin C (MMC) has shown promising insights in the inhibition of pterygium recurrence, it needs to be investigated in more details in molecular pathways to present adjuvant target therapy. In this study, we aimed to evaluate the expression of and then illustrate the role of signaling pathways on EMT in pterygium. Using real-time polymerase chain reaction, the twist-related protein 1 (TWIST1) expression was compared in primary pterygium and normal conjunctiva. This study assessed the mRNA expression, as well as the association between the clinicopathological indices and the gene expression level. The expression level of TWIST1 was overexpressed in 36% of our cohort ( n = 76). There was a significant positive correlation between recurrence with grade T, grade V and a significant negative correlation with growth activity. Our vast literature review on different signaling pathways in pterygium showed that EMT has centralization role in recurrence of this disease. Our data confirmed that EMT is important in the recurrence of pterygium samples and different signaling pathways end up activating the EMT markers. It is suggested to evaluate the environmental factors and their correlation with molecular markers to select favorable treatment for this kind of diseases.
(© 2019 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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