Enhancement of the enzymatic cellulose saccharification by Penicillium verruculosum multienzyme cocktails containing homologously overexpressed lytic polysaccharide monooxygenase.

Autor: Semenova MV; Federal Research Centre 'Fundamentals of Biotechnology' of the Russian Academy of Sciences, Leninsky Pr. 33/2, Moscow, 119071, Russia., Gusakov AV; Federal Research Centre 'Fundamentals of Biotechnology' of the Russian Academy of Sciences, Leninsky Pr. 33/2, Moscow, 119071, Russia. avgusakov@enzyme.chem.msu.ru.; Department of Chemistry, M. V. Lomonosov Moscow State University, Vorobyovy Gory 1/11, Moscow, 119991, Russia. avgusakov@enzyme.chem.msu.ru., Volkov PV; Federal Research Centre 'Fundamentals of Biotechnology' of the Russian Academy of Sciences, Leninsky Pr. 33/2, Moscow, 119071, Russia., Matys VY; G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia., Nemashkalov VA; G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia., Telitsin VD; Department of Chemistry, M. V. Lomonosov Moscow State University, Vorobyovy Gory 1/11, Moscow, 119991, Russia., Rozhkova AM; Federal Research Centre 'Fundamentals of Biotechnology' of the Russian Academy of Sciences, Leninsky Pr. 33/2, Moscow, 119071, Russia.; Department of Chemistry, M. V. Lomonosov Moscow State University, Vorobyovy Gory 1/11, Moscow, 119991, Russia., Sinitsyn AP; Federal Research Centre 'Fundamentals of Biotechnology' of the Russian Academy of Sciences, Leninsky Pr. 33/2, Moscow, 119071, Russia.; Department of Chemistry, M. V. Lomonosov Moscow State University, Vorobyovy Gory 1/11, Moscow, 119991, Russia.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2019 Apr; Vol. 46 (2), pp. 2363-2370. Date of Electronic Publication: 2019 Feb 15.
DOI: 10.1007/s11033-019-04693-y
Abstrakt: The gene lpmo1 encoding Penicillium verruculosum lytic polysaccharide monooxygenase (PvLPMO9A) was sequenced and homologously overexpressed in P. verruculosum B1-537 (ΔniaD) auxotrophic strain under the control of the cbh1 gene promoter in combination with either the cbh1 signal sequence (sCBH1-X series of samples) or the native lpmo1 signal sequence (sLPMO1-X series). Three enzyme samples of the sCBH1-X series were characterized by a lower overall content of cellobiohydrolases (CBHs: 26-45%) but slightly higher content of endoglucanases (EGs: 17-23%) relative to the reference B1-537 preparation (60% of CBHs and 14% of EGs), while the PvLPMO9A content in them made up 9-21% of the total secreted protein. The PvLPMO9A content in four enzyme preparations of the sLPMO1-X series was much higher (30-57%), however the portion of CBHs in most of them (except for sLPMO1-8) decreased even to a greater extent (to 21-42%) than in the samples of the sCBH1-X series. Two enzyme preparations (sCBH1-8 and sLPMO1-8), in which the content of cellulases was substantially retained and the portion of PvLPMO9A was 9-30%, demonstrated the increased yields of reducing sugars in 48-h saccharification of Avicel and milled aspen wood: 19-31 and 11-26%, respectively, compared to the reference cellulase cocktail.
Databáze: MEDLINE
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