Graphene oxide and gold nanoparticle based dual platform with short DNA probe for the PCR free DNA biosensing using surface-enhanced Raman scattering.

Autor: Khalil I; Nanotechnology & Catalysis Research Centre (NANOCAT), Institute of Graduate Studies, University of Malaya, Kuala Lumpur 50603, Malaysia., Yehye WA; Nanotechnology & Catalysis Research Centre (NANOCAT), Institute of Graduate Studies, University of Malaya, Kuala Lumpur 50603, Malaysia. Electronic address: wdabdoub@um.edu.my., Julkapli NM; Nanotechnology & Catalysis Research Centre (NANOCAT), Institute of Graduate Studies, University of Malaya, Kuala Lumpur 50603, Malaysia., Rahmati S; Nanotechnology & Catalysis Research Centre (NANOCAT), Institute of Graduate Studies, University of Malaya, Kuala Lumpur 50603, Malaysia., Sina AA; Centre for Personalised Nanomedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Corner of College and Cooper Roads (Bldg 75), Brisbane QLD 4072, Australia., Basirun WJ; Nanotechnology & Catalysis Research Centre (NANOCAT), Institute of Graduate Studies, University of Malaya, Kuala Lumpur 50603, Malaysia; Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia., Johan MR; Nanotechnology & Catalysis Research Centre (NANOCAT), Institute of Graduate Studies, University of Malaya, Kuala Lumpur 50603, Malaysia.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2019 Apr 15; Vol. 131, pp. 214-223. Date of Electronic Publication: 2019 Feb 19.
DOI: 10.1016/j.bios.2019.02.028
Abstrakt: Surface-enhanced Raman scattering (SERS) based DNA biosensors have considered as excellent, fast and ultrasensitive sensing technique which relies on the fingerprinting ability to produce molecule specific distinct spectra. Unlike conventional fluorescence based strategies SERS provides narrow spectral bandwidths, fluorescence quenching and multiplexing ability, and fitting attribute with short length probe DNA sequences. Herein, we report a novel and PCR free SERS based DNA detection strategy involving dual platforms and short DNA probes for the detection of endangered species, Malayan box turtle (MBT) (Cuora amboinensis). In this biosensing feature, the detection is based on the covalent linking of the two platforms involving graphene oxide-gold nanoparticles (GO-AuNPs) functionalized with capture probe 1 and gold nanoparticles (AuNPs) modified with capture probe 2 and Raman dye (Cy3) via hybridization with the corresponding target sequences. Coupling of the two platforms generates locally enhanced electromagnetic field 'hot spot', formed at the junctions and interstitial crevices of the nanostructures and consequently provide significant amplification of the SERS signal. Therefore, employing the two SERS active substrates and short-length probe DNA sequences, we have managed to improve the sensitivity of the biosensors to achieve a lowest limit of detection (LOD) as low as 10 fM. Furthermore, the fabricated biosensor exhibited sensitivity even for single nucleotide base-mismatch in the target DNA as well as showed excellent performance to discriminate closely related six non-target DNA sequences. Although the developed SERS biosensor would be an attractive platform for the authentication of MBT from diverse samples including forensic and/or archaeological specimens, it could have universal application for detecting gene specific biomarkers for many diseases including cancer.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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