Seasonal variation in equine follicular fluid proteome.

Autor: Dutra GA; Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA.; Department of Reproduction and Animal Evaluation, Federal Rural University of Rio de Janeiro, Seropédica, Brazil., Ishak GM; Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA.; Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq., Pechanova O; Institute for Genomics, Biocomputing and Bioinformatics, University, Mississippi State, Oxford, MS, USA., Pechan T; Institute for Genomics, Biocomputing and Bioinformatics, University, Mississippi State, Oxford, MS, USA., Peterson DG; Institute for Genomics, Biocomputing and Bioinformatics, University, Mississippi State, Oxford, MS, USA., Jacob JCF; Department of Reproduction and Animal Evaluation, Federal Rural University of Rio de Janeiro, Seropédica, Brazil., Willard ST; Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, 4025 Wise Center, PO Box 9815, Mississippi State, MS, 39762, USA., Ryan PL; Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, 4025 Wise Center, PO Box 9815, Mississippi State, MS, 39762, USA., Gastal EL; Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA., Feugang JM; Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, 4025 Wise Center, PO Box 9815, Mississippi State, MS, 39762, USA. jn181@ads.msstate.edu.
Jazyk: angličtina
Zdroj: Reproductive biology and endocrinology : RB&E [Reprod Biol Endocrinol] 2019 Mar 06; Vol. 17 (1), pp. 29. Date of Electronic Publication: 2019 Mar 06.
DOI: 10.1186/s12958-019-0473-z
Abstrakt: Background: Proteomic studies of follicular fluid (FF) exist for several species, including the horse; however, the seasonal influence on FF proteome has not been explored in livestock. The application of high-throughput proteomics of FF in horse has the potential to identify seasonal variations of proteins involved in follicle and oocyte growth.
Methods: This study (i) profiles the proteomes of equine FF collected from dominant growing follicles during the spring anovulatory season (SAN), and spring (SOV), summer (SUM), and fall (FOV) ovulatory seasons; and (ii) identifies season-dependent regulatory networks and associated key proteins.
Results: Regardless of season, a total of 90 proteins were identified in FF, corresponding to 63, 72, 69, and 78 proteins detected in the SAN, SOV, SUM, and FOV seasons, respectively. Fifty-two proteins were common to all seasons, a total of 13 were unique to either season, and 25 were shared between two seasons or more. Protein-to-protein interaction (PPI) analysis indicated the likely critical roles of plasminogen in the SAN season, the prothrombin/plasminogen combination in SUM, and plasminogen/complement C3 in both SOV and FOV seasons. The apolipoprotein A1 appeared crucial in all seasons. The present findings show that FF proteome of SUM differs from other seasons, with FF having high fluidity (low viscosity).
Conclusions: The balance between the FF contents in prothrombin, plasminogen, and coagulation factor XII proteins favoring FF fluidity may be crucial at the peak of the ovulatory season (SUM) and may explain the reported lower incidence of hemorrhagic anovulatory follicles during the SUM season.
Databáze: MEDLINE
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